β-Galactosidase-immobilised microreactor fabricated using a novel technique for enzyme immobilisation and its application for continuous synthesis of lactulose

Yoon Seok Song, Hyun Yong Shin, Jin Young Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

In this study, several surface functionalisation techniques were used to immobilise β-galactosidase in a microreactor. β-Galactosidase was pretreated with lactose before immobilisation, and functionalised multi-walled carbon nanotubes (MWNTs), DNA-wrapped single-walled carbon nanotubes and glutaraldehyde as a linker were used to immobilise β-galactosidase on a microchannel surface. When functionalised MWNTs were used as a linker for immobilisation of pretreated β-galactosidase, the enzyme microreactor showed the highest performance. The K m(app) and V max were 2.84 mM and 0.52 mM/min, respectively, and the conversion of o-nitrophenyl-β-d-galactopyranoside (ONPG) reached 78.3% during the continuous flow reaction at a flow rate of 2.5 μL/min. In an enzyme microreactor, continuous synthesis of lactulose was performed, and the lactulose concentration was maintained at about 1.29 g/L for 48 h.

Original languageEnglish
Pages (from-to)611-617
Number of pages7
JournalFood Chemistry
Volume133
Issue number3
DOIs
Publication statusPublished - 2012 Aug 1

Keywords

  • Enzyme microreactor
  • Functionalised multi-walled carbon nanotubes
  • Lactulose
  • Pretreatment
  • β-Galactosidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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