β-PIX is critical for transplanted mesenchymal stromal cell migration

Seong Ho Koh, Yong Min Huh, Min Young Noh, Hyun Young Kim, Kyung Suk Kim, Eun Sook Lee, Hyun Ju Ko, Goang Won Cho, A. Rum Yoo, Ho Taek Song, Sejin Hwang, Kwangyeol Lee, Seungjoo Haam, Joseph A. Frank, Jin Suck Suh, Seung Hyun Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Bone marrow-derived mesenchymal stromal cells (MSCs) have been used successfully as a source of stem cells for treating neurodegenerative diseases. However, for reasons that are not clear, autologous MSC transplants have not yielded successful results in human trials. To test one possible reason, we compared the migratory ability of MSCs from amyotrophic lateral sclerosis (ALS) patients with those of healthy controls. We found that MSCs derived from ALS patients (ALS-MSCs) had a reduced ability to migrate, which may explain why autologous transplantation is not successful. We also found that expression of one of the intracellular factors implicated in migration, β-PIX, was significantly reduced in ALS-MSCs compared with healthy stem cells. Restoration of β-PIX expression by genetic manipulation restored the migratory ability of ALS-MSCs, and inhibition of β-PIX expression with shRNA reduced the migration of healthy MSCs. We suggest that transplantation of allogeneic or genetically modified autologous stem cells might be a more promising strategy for ALS patients than transplantation of autologous stem cells.

Original languageEnglish
Pages (from-to)1989-1999
Number of pages11
JournalStem Cells and Development
Volume21
Issue number11
DOIs
Publication statusPublished - 2012 Jul 20

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

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  • Cite this

    Koh, S. H., Huh, Y. M., Noh, M. Y., Kim, H. Y., Kim, K. S., Lee, E. S., Ko, H. J., Cho, G. W., Yoo, A. R., Song, H. T., Hwang, S., Lee, K., Haam, S., Frank, J. A., Suh, J. S., & Kim, S. H. (2012). β-PIX is critical for transplanted mesenchymal stromal cell migration. Stem Cells and Development, 21(11), 1989-1999. https://doi.org/10.1089/scd.2011.0430