Abstract
γ-Glutamyltransferase (GGT) activity is known to increase during hepatocarcinogenesis induced by chemical carcinogens. To understand the possible significance of GGT, we have investigated GGT activities in hepatocytes cultured with or without metabolic inhibitors. Primary cultures of adult rat hepatocytes were established and the cell number, [3H]thymidine incorporation and GGT activity were measured at various points of hepatocyte proliferation. When insulin (0.1 μM) and EGF (0.1 μg/ml) were added to the culture, the initially low hepatocyte density (5-6 x 104 cells/cm2) has increased 1.5-fold after 32 h of incubation. The change of GGT activity was correlated with that of [3H]thymidine incorporation: both the GGT activity and the [3H]thymidine incorporation have increased 2.6-fold and 2.5-fold, respectively, after 3 h of incubation, and then decreased rapidly followed by another increase after 24 h. The effect of inhibitors, such as serine-borate complex and acivicin for GGT during hepatocyte proliferation was also investigated. Serine-borate complex (reversible inhibitor) and acivicin (irreversible inhibitor) inhibited proliferation of hepatocyte in a dose-dependant manner: At the concentration of 1 mM serine-borate complex or 0.5 mM acivicin, hepatocyte proliferation was completely abolished, while GGT activity decreased slightly even at the 5 mM serine-borate complex. When hepatocytes were initially cultured in the presence of the growth factor with 5 mM serine-borate complex for 24 h, and then continued to culture further in the absence of the inhibitor, the cells restored proliferating capability completely, indicating the reversibility of the inhibitor. In conclusion, we have demonstrated that during hepatocyte proliferation increase in GGT activity is temporarily correlated with DNA synthesis, suggesting that GGT may play a role in hepatocyte growth.
| Original language | English |
|---|---|
| Pages (from-to) | 17-23 |
| Number of pages | 7 |
| Journal | Experimental and Molecular Medicine |
| Volume | 28 |
| Issue number | 1 |
| Publication status | Published - 1996 Jun 17 |
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Keywords
- γ-glutamyltransferase
- Acivicin
- Hepatocyte proliferation
- Serine berate complex
ASJC Scopus subject areas
- Biochemistry
- Genetics
Cite this
γ-Glutamyltransferase in adult rat hepatocyte during proliferation. / Park, Yun Gyu; Kim, Meyoung-Kon; Sohn, Jeongwon; Park, Gil-Hong; Ryu, Chong Kun.
In: Experimental and Molecular Medicine, Vol. 28, No. 1, 17.06.1996, p. 17-23.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - γ-Glutamyltransferase in adult rat hepatocyte during proliferation
AU - Park, Yun Gyu
AU - Kim, Meyoung-Kon
AU - Sohn, Jeongwon
AU - Park, Gil-Hong
AU - Ryu, Chong Kun
PY - 1996/6/17
Y1 - 1996/6/17
N2 - γ-Glutamyltransferase (GGT) activity is known to increase during hepatocarcinogenesis induced by chemical carcinogens. To understand the possible significance of GGT, we have investigated GGT activities in hepatocytes cultured with or without metabolic inhibitors. Primary cultures of adult rat hepatocytes were established and the cell number, [3H]thymidine incorporation and GGT activity were measured at various points of hepatocyte proliferation. When insulin (0.1 μM) and EGF (0.1 μg/ml) were added to the culture, the initially low hepatocyte density (5-6 x 104 cells/cm2) has increased 1.5-fold after 32 h of incubation. The change of GGT activity was correlated with that of [3H]thymidine incorporation: both the GGT activity and the [3H]thymidine incorporation have increased 2.6-fold and 2.5-fold, respectively, after 3 h of incubation, and then decreased rapidly followed by another increase after 24 h. The effect of inhibitors, such as serine-borate complex and acivicin for GGT during hepatocyte proliferation was also investigated. Serine-borate complex (reversible inhibitor) and acivicin (irreversible inhibitor) inhibited proliferation of hepatocyte in a dose-dependant manner: At the concentration of 1 mM serine-borate complex or 0.5 mM acivicin, hepatocyte proliferation was completely abolished, while GGT activity decreased slightly even at the 5 mM serine-borate complex. When hepatocytes were initially cultured in the presence of the growth factor with 5 mM serine-borate complex for 24 h, and then continued to culture further in the absence of the inhibitor, the cells restored proliferating capability completely, indicating the reversibility of the inhibitor. In conclusion, we have demonstrated that during hepatocyte proliferation increase in GGT activity is temporarily correlated with DNA synthesis, suggesting that GGT may play a role in hepatocyte growth.
AB - γ-Glutamyltransferase (GGT) activity is known to increase during hepatocarcinogenesis induced by chemical carcinogens. To understand the possible significance of GGT, we have investigated GGT activities in hepatocytes cultured with or without metabolic inhibitors. Primary cultures of adult rat hepatocytes were established and the cell number, [3H]thymidine incorporation and GGT activity were measured at various points of hepatocyte proliferation. When insulin (0.1 μM) and EGF (0.1 μg/ml) were added to the culture, the initially low hepatocyte density (5-6 x 104 cells/cm2) has increased 1.5-fold after 32 h of incubation. The change of GGT activity was correlated with that of [3H]thymidine incorporation: both the GGT activity and the [3H]thymidine incorporation have increased 2.6-fold and 2.5-fold, respectively, after 3 h of incubation, and then decreased rapidly followed by another increase after 24 h. The effect of inhibitors, such as serine-borate complex and acivicin for GGT during hepatocyte proliferation was also investigated. Serine-borate complex (reversible inhibitor) and acivicin (irreversible inhibitor) inhibited proliferation of hepatocyte in a dose-dependant manner: At the concentration of 1 mM serine-borate complex or 0.5 mM acivicin, hepatocyte proliferation was completely abolished, while GGT activity decreased slightly even at the 5 mM serine-borate complex. When hepatocytes were initially cultured in the presence of the growth factor with 5 mM serine-borate complex for 24 h, and then continued to culture further in the absence of the inhibitor, the cells restored proliferating capability completely, indicating the reversibility of the inhibitor. In conclusion, we have demonstrated that during hepatocyte proliferation increase in GGT activity is temporarily correlated with DNA synthesis, suggesting that GGT may play a role in hepatocyte growth.
KW - γ-glutamyltransferase
KW - Acivicin
KW - Hepatocyte proliferation
KW - Serine berate complex
UR - http://www.scopus.com/inward/record.url?scp=0030014580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030014580&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0030014580
VL - 28
SP - 17
EP - 23
JO - Experimental and Molecular Medicine
JF - Experimental and Molecular Medicine
SN - 1226-3613
IS - 1
ER -