1-deoxynojirimycin isolated from a Bacillus subtilis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes

Seung Min ee, Hyun Ju Do, Min Jeong Shin, Su Il Seong, Kyo Yeol Hwang, Jae Yeon Lee, Ohsuk Kwon, Taewon Jin, Ji Hyung Chung

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.

Original languageEnglish
Pages (from-to)637-643
Number of pages7
JournalJournal of microbiology and biotechnology
Volume23
Issue number5
DOIs
Publication statusPublished - 2013 Mar 12

Keywords

  • 1-deoxynojirimycin
  • AMPK
  • Adipocytes
  • Adiponectin
  • Adipor

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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