1-deoxynojirimycin isolated from a Bacillus subtilis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes

Seung Min ee, Hyun Ju Do, Min-Jeong Shin, Su Il Seong, Kyo Yeol Hwang, Jae Yeon Lee, Ohsuk Kwon, Taewon Jin, Ji Hyung Chung

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.

Original languageEnglish
Pages (from-to)637-643
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume23
Issue number5
DOIs
Publication statusPublished - 2013 Mar 12

Fingerprint

1-Deoxynojirimycin
Facilitative Glucose Transport Proteins
Adiponectin
Bacillus subtilis
Adipocytes
Adiponectin Receptors
Glucose
Adenosine
Protein Kinases
Messenger RNA
Poisons
Deoxyglucose
Transcriptional Activation
Insulin Resistance
Blood Glucose
Adipose Tissue
Proteins
Phosphorylation
Cell Membrane

Keywords

  • 1-deoxynojirimycin
  • Adipocytes
  • Adiponectin
  • Adipor
  • AMPK

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

1-deoxynojirimycin isolated from a Bacillus subtilis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes. / ee, Seung Min; Do, Hyun Ju; Shin, Min-Jeong; Seong, Su Il; Hwang, Kyo Yeol; Lee, Jae Yeon; Kwon, Ohsuk; Jin, Taewon; Chung, Ji Hyung.

In: Journal of Microbiology and Biotechnology, Vol. 23, No. 5, 12.03.2013, p. 637-643.

Research output: Contribution to journalArticle

ee, Seung Min ; Do, Hyun Ju ; Shin, Min-Jeong ; Seong, Su Il ; Hwang, Kyo Yeol ; Lee, Jae Yeon ; Kwon, Ohsuk ; Jin, Taewon ; Chung, Ji Hyung. / 1-deoxynojirimycin isolated from a Bacillus subtilis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes. In: Journal of Microbiology and Biotechnology. 2013 ; Vol. 23, No. 5. pp. 637-643.
@article{b727347e2cb84d909a7bad65baa7c9cb,
title = "1-deoxynojirimycin isolated from a Bacillus subtilis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes",
abstract = "We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.",
keywords = "1-deoxynojirimycin, Adipocytes, Adiponectin, Adipor, AMPK",
author = "ee, {Seung Min} and Do, {Hyun Ju} and Min-Jeong Shin and Seong, {Su Il} and Hwang, {Kyo Yeol} and Lee, {Jae Yeon} and Ohsuk Kwon and Taewon Jin and Chung, {Ji Hyung}",
year = "2013",
month = "3",
day = "12",
doi = "10.4014/jmb.1209.09043",
language = "English",
volume = "23",
pages = "637--643",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "5",

}

TY - JOUR

T1 - 1-deoxynojirimycin isolated from a Bacillus subtilis stimulates adiponectin and GLUT4 expressions in 3T3-L1 adipocytes

AU - ee, Seung Min

AU - Do, Hyun Ju

AU - Shin, Min-Jeong

AU - Seong, Su Il

AU - Hwang, Kyo Yeol

AU - Lee, Jae Yeon

AU - Kwon, Ohsuk

AU - Jin, Taewon

AU - Chung, Ji Hyung

PY - 2013/3/12

Y1 - 2013/3/12

N2 - We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.

AB - We have demonstrated that 1-deoxynojirimycin (DNJ) isolated from Bacillus subtilis MORI could enhance the levels of adiponectin and its receptors in differentiated 3T3-L1 adipocytes, which has been shown to be effective in lowering blood glucose levels and enhancing insulin sensitivity. DNJ was not toxic to differentiated 3T3-L1 adipocytes for up to a concentration of 5 μM. In terms of expression levels of adiponectin and its receptors (AdipoR1 and AdipoR2), DNJ in concentrations as low as 0.5 μM elevated both mRNA and protein levels of adiponectin and transcript levels of AdipoR1 and AdipoR2. In addition, DNJ increased phosphorylation of 5' adenosine monophosphateactivated protein kinase (AMPK) in a statistically significant manner. Finally, treatment with DNJ resulted in increased mRNA expression of glucose transporter 4 (GLUT4), which encodes for a glucose transporter, along with a significant increase in glucose uptake into the adipocytes based on results of a 2-deoxy-D-[3H] glucose uptake assay. Our findings indicate that DNJ may greatly facilitate glucose uptake into adipose tissues by increasing the action of adiponectin via its up-regulated expression as well as its receptor genes. In addition, the glucose-lowering effects of DNJ may be achieved by an increased abundance of GLUT4 protein in the plasma membrane, as a consequence of the increased transcript levels of the GLUT4 gene and the activation of AMPK.

KW - 1-deoxynojirimycin

KW - Adipocytes

KW - Adiponectin

KW - Adipor

KW - AMPK

UR - http://www.scopus.com/inward/record.url?scp=84877357123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877357123&partnerID=8YFLogxK

U2 - 10.4014/jmb.1209.09043

DO - 10.4014/jmb.1209.09043

M3 - Article

C2 - 23648852

AN - SCOPUS:84877357123

VL - 23

SP - 637

EP - 643

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 5

ER -