1H nuclear magnetic resonance based metabolic urinary profiling of patients with ischemic heart failure

Seok Min Kang, Jong Chul Park, Min Jeong Shin, Hyeran Lee, Jaewon Oh, Do Hyun Ryu, Geum Sook Hwang, Ji Hyung Chung

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Objectives: We sought to identify metabolic pathways characterizing human heart failure (HF) using 1NMR based urinary metabolomic analysis in conjunction with multivariate statistics. Design and methods: Patients with systolic HF of ischemic origin (n = 15) and healthy controls (n. = 20) participated in this study. Patients with type 2 diabetes mellitus were excluded. Results: The results showed that the urine of the HF patients had higher levels of metabolites for acetate (p < 0.05) and acetone (p < 0.01) compared to the healthy controls. In addition, there was a perturbation in methylmalonate metabolism as shown by increased urinary levels of methylmalonic acid (p < 0.001) in the HF patients. HF patients also had increased urinary levels of cytosine (p < 0.01) and phenylacetylglycine (p < 0.01) and decreased 1-methylnicotinamide (p < 0.05) compared to healthy controls. Conclusions: TCA cycle metabolites and fatty acid metabolism were modified in the HF patients, indicating altered energy metabolism. Moreover, perturbations of metabolism in nucleotide and methylmalonate were observed.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalClinical Biochemistry
Volume44
Issue number4
DOIs
Publication statusPublished - 2011 Mar

Keywords

  • Citric acid cycle
  • Heart failure
  • Metabolism
  • Methylmalonic acid
  • Phenylacetylglycine

ASJC Scopus subject areas

  • Clinical Biochemistry

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