1p36.22 region containing PGD gene is frequently gained in human cervical cancer

Maria Lee, Eun Sook Nam, Seung Hyun Jung, Soon Young Kim, Sung Jong Lee, Joo Hee Yoon, Nak Woo Lee, Seob Jeon, Joong Sub Choi, Chi Heum Cho, Yong Moon, Yeun Jun Chung, Yongil Kwon

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aim: To identify commonly occurring DNA copy number alterations in Korean cervical cancers. Methods: DNA copy number alteration was screened by whole-genome array comparative genomic hybridization (CGH) analysis. For the array CGH discovery, genomic DNA from five cervical cancers and 10 normal cervical tissues were examined. For the independent validation of the most significant chromosomal alteration (1p36.22, PGD gene), 40 formalin-fixed paraffin-embedded cervical tissue samples were collected; 10 of them were used for quantitative polymerase chain reaction and the other 30 samples were used for immunohistochemical analysis. Chromosomal segments differently distributed between cancers and normal controls were determined to be recurrently altered regions (RAR). Results: A total of 13 RAR (11 RAR losses and two RAR gains) were defined in this study. Of the 13 cervical cancer-specific RAR, RAR gain in the 1p36.22 locus where the PGD gene is located was the most commonly detected in cancers (P = 0.004). In the quantitative polymerase chain reaction replication, copy number gain of the PGD gene was consistently identified in cervical cancers but not in the normal tissues (P = 0.02). In immunohistochemical analysis, PGD expression was significantly higher in cervical cancers than normal tissues (P = 0.02). Conclusion: Our results will be helpful to understand cervical carcinogenesis, and the PGD gene can be a useful biomarker of cervical cancer.

Original languageEnglish
Pages (from-to)545-553
Number of pages9
JournalJournal of Obstetrics and Gynaecology Research
Volume40
Issue number2
DOIs
Publication statusPublished - 2014 Feb

Keywords

  • Array comparative genomic hybridization
  • Cervical cancer
  • Copy number alteration
  • PGD
  • Recurrently altered region

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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  • Cite this

    Lee, M., Nam, E. S., Jung, S. H., Kim, S. Y., Lee, S. J., Yoon, J. H., Lee, N. W., Jeon, S., Choi, J. S., Cho, C. H., Moon, Y., Chung, Y. J., & Kwon, Y. (2014). 1p36.22 region containing PGD gene is frequently gained in human cervical cancer. Journal of Obstetrics and Gynaecology Research, 40(2), 545-553. https://doi.org/10.1111/jog.12193