2 X 3 array oxygen sensor for measuring cellular respiration level

Jungil Park, Sunyoung Ahn, Youngmi Kim Pak, James Jungho Pak

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper presents a microfabricated 2 × 3 array oxygen sensor integrated with heaters and temperature sensors for measuring cellular respiration level in a solution containing cells. The proposed oxygen sensor consists of 2 x 3 array Clarktype sensors, heaters, and temperature sensors on the substrate. Pt was used for temperature sensors, working electrode and counter electrode of Clark-type sensor because it has very stable physical and chemical properties and resistance linearity to temperature. Ag/AgCI was formed by electrochemical method on patterned Ag and used as reference electrode. Heaters as Au were patterned on the opposite side of the substrate. PDMS was coated on the acrylic plate by using spin coater, and PDMS thickness is about 18pm. It used as an oxygen permeable membrane of Clark-type sensor. The peak oxygen reduction current of the fabricated sensor was measured at -0.8 V by using cyclic voltammetry, and the 90% response time was about 10 sec from full-oxygen state to zero-oxygen state. The fabricated sensor showed good reproducibility for I hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The temperature sensor showed an excellent linearity with a correlation coefficient of 0.999 from 32°C to 55°C.

Original languageEnglish
Title of host publication4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
Pages1054-1057
Number of pages4
DOIs
Publication statusPublished - 2009 Oct 12
Event4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China
Duration: 2009 Jan 52009 Jan 8

Other

Other4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
CountryChina
CityShenzhen
Period09/1/509/1/8

Fingerprint

Oxygen sensors
Temperature sensors
Oxygen
Sensors
Electrodes
Oxygen permeable membranes
Chemical resistance
Sensor arrays
Substrates
Chemical properties
Cyclic voltammetry
Acrylics
Physical properties

Keywords

  • Cellular respiration
  • Clark-type sensor
  • Microfabrication
  • PDMS
  • Potentiostat

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Park, J., Ahn, S., Pak, Y. K., & Pak, J. J. (2009). 2 X 3 array oxygen sensor for measuring cellular respiration level. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 (pp. 1054-1057). [5068754] https://doi.org/10.1109/NEMS.2009.5068754

2 X 3 array oxygen sensor for measuring cellular respiration level. / Park, Jungil; Ahn, Sunyoung; Pak, Youngmi Kim; Pak, James Jungho.

4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 1054-1057 5068754.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Park, J, Ahn, S, Pak, YK & Pak, JJ 2009, 2 X 3 array oxygen sensor for measuring cellular respiration level. in 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009., 5068754, pp. 1054-1057, 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009, Shenzhen, China, 09/1/5. https://doi.org/10.1109/NEMS.2009.5068754
Park J, Ahn S, Pak YK, Pak JJ. 2 X 3 array oxygen sensor for measuring cellular respiration level. In 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 1054-1057. 5068754 https://doi.org/10.1109/NEMS.2009.5068754
Park, Jungil ; Ahn, Sunyoung ; Pak, Youngmi Kim ; Pak, James Jungho. / 2 X 3 array oxygen sensor for measuring cellular respiration level. 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. pp. 1054-1057
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AB - This paper presents a microfabricated 2 × 3 array oxygen sensor integrated with heaters and temperature sensors for measuring cellular respiration level in a solution containing cells. The proposed oxygen sensor consists of 2 x 3 array Clarktype sensors, heaters, and temperature sensors on the substrate. Pt was used for temperature sensors, working electrode and counter electrode of Clark-type sensor because it has very stable physical and chemical properties and resistance linearity to temperature. Ag/AgCI was formed by electrochemical method on patterned Ag and used as reference electrode. Heaters as Au were patterned on the opposite side of the substrate. PDMS was coated on the acrylic plate by using spin coater, and PDMS thickness is about 18pm. It used as an oxygen permeable membrane of Clark-type sensor. The peak oxygen reduction current of the fabricated sensor was measured at -0.8 V by using cyclic voltammetry, and the 90% response time was about 10 sec from full-oxygen state to zero-oxygen state. The fabricated sensor showed good reproducibility for I hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The temperature sensor showed an excellent linearity with a correlation coefficient of 0.999 from 32°C to 55°C.

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