Abstract
This paper presents a microfabricated 2 × 3 array oxygen sensor integrated with heaters and temperature sensors for measuring cellular respiration level in a solution containing cells. The proposed oxygen sensor consists of 2 x 3 array Clarktype sensors, heaters, and temperature sensors on the substrate. Pt was used for temperature sensors, working electrode and counter electrode of Clark-type sensor because it has very stable physical and chemical properties and resistance linearity to temperature. Ag/AgCI was formed by electrochemical method on patterned Ag and used as reference electrode. Heaters as Au were patterned on the opposite side of the substrate. PDMS was coated on the acrylic plate by using spin coater, and PDMS thickness is about 18pm. It used as an oxygen permeable membrane of Clark-type sensor. The peak oxygen reduction current of the fabricated sensor was measured at -0.8 V by using cyclic voltammetry, and the 90% response time was about 10 sec from full-oxygen state to zero-oxygen state. The fabricated sensor showed good reproducibility for I hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The temperature sensor showed an excellent linearity with a correlation coefficient of 0.999 from 32°C to 55°C.
| Original language | English |
|---|---|
| Title of host publication | 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 |
| Pages | 1054-1057 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2009 Oct 12 |
| Event | 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 - Shenzhen, China Duration: 2009 Jan 5 → 2009 Jan 8 |
Other
| Other | 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009 |
|---|---|
| Country | China |
| City | Shenzhen |
| Period | 09/1/5 → 09/1/8 |
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Keywords
- Cellular respiration
- Clark-type sensor
- Microfabrication
- PDMS
- Potentiostat
ASJC Scopus subject areas
- Electrical and Electronic Engineering
Cite this
2 X 3 array oxygen sensor for measuring cellular respiration level. / Park, Jungil; Ahn, Sunyoung; Pak, Youngmi Kim; Pak, James Jungho.
4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009. 2009. p. 1054-1057 5068754.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - 2 X 3 array oxygen sensor for measuring cellular respiration level
AU - Park, Jungil
AU - Ahn, Sunyoung
AU - Pak, Youngmi Kim
AU - Pak, James Jungho
PY - 2009/10/12
Y1 - 2009/10/12
N2 - This paper presents a microfabricated 2 × 3 array oxygen sensor integrated with heaters and temperature sensors for measuring cellular respiration level in a solution containing cells. The proposed oxygen sensor consists of 2 x 3 array Clarktype sensors, heaters, and temperature sensors on the substrate. Pt was used for temperature sensors, working electrode and counter electrode of Clark-type sensor because it has very stable physical and chemical properties and resistance linearity to temperature. Ag/AgCI was formed by electrochemical method on patterned Ag and used as reference electrode. Heaters as Au were patterned on the opposite side of the substrate. PDMS was coated on the acrylic plate by using spin coater, and PDMS thickness is about 18pm. It used as an oxygen permeable membrane of Clark-type sensor. The peak oxygen reduction current of the fabricated sensor was measured at -0.8 V by using cyclic voltammetry, and the 90% response time was about 10 sec from full-oxygen state to zero-oxygen state. The fabricated sensor showed good reproducibility for I hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The temperature sensor showed an excellent linearity with a correlation coefficient of 0.999 from 32°C to 55°C.
AB - This paper presents a microfabricated 2 × 3 array oxygen sensor integrated with heaters and temperature sensors for measuring cellular respiration level in a solution containing cells. The proposed oxygen sensor consists of 2 x 3 array Clarktype sensors, heaters, and temperature sensors on the substrate. Pt was used for temperature sensors, working electrode and counter electrode of Clark-type sensor because it has very stable physical and chemical properties and resistance linearity to temperature. Ag/AgCI was formed by electrochemical method on patterned Ag and used as reference electrode. Heaters as Au were patterned on the opposite side of the substrate. PDMS was coated on the acrylic plate by using spin coater, and PDMS thickness is about 18pm. It used as an oxygen permeable membrane of Clark-type sensor. The peak oxygen reduction current of the fabricated sensor was measured at -0.8 V by using cyclic voltammetry, and the 90% response time was about 10 sec from full-oxygen state to zero-oxygen state. The fabricated sensor showed good reproducibility for I hour with 3.15nA standard deviation of 108.2nA mean in the full-oxygen state and 0.14nA standard deviation of 1.33nA mean in the zero-oxygen state. Also, the fabricated sensor showed a good linearity with a correlation coefficient of 0.997. The temperature sensor showed an excellent linearity with a correlation coefficient of 0.999 from 32°C to 55°C.
KW - Cellular respiration
KW - Clark-type sensor
KW - Microfabrication
KW - PDMS
KW - Potentiostat
UR - http://www.scopus.com/inward/record.url?scp=70349664763&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349664763&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2009.5068754
DO - 10.1109/NEMS.2009.5068754
M3 - Conference contribution
SN - 9781424446308
SP - 1054
EP - 1057
BT - 4th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2009
ER -