2A-linked bi-, tri-, and quad-cistrons for the stepwise biosynthesis of β-carotene, zeaxanthin, and ketocarotenoids in rice endosperm

Ye Sol Jeong, Hyung Keun Ku, Young Joo Jung, Jae Kwang Kim, Kyoung Bok Lee, Ju Kon Kim, Sun Hyung Lim, Dongho Lee, Sun Hwa Ha

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Foot-and-mouth disease virus (FMDV) 2A constructs have been successfully used for the production of “Golden Rice”, a β-carotene producing rice strain. However, to allay public fears and opposition to plants carrying a mammalian pathogenic viral sequence, 2A-like synthetic sequences from Thosea asigna virus and Infectious myonecrosis virus were used to coordinate the coexpression of carotenoid biosynthetic genes. Here, up to four carotenogenic genes encoding PSY, CRTI, BCH and BKT were concatenated and produced β-carotene, zeaxanthin, and ketocarotenoids (astaxanthin and adonixanthin) in transgenic rice seeds displaying color variation due to the difference in carotenoid content and composition.

Original languageEnglish
Article numbere00166
JournalMetabolic Engineering Communications
Volume12
DOIs
Publication statusPublished - 2021 Jun

Keywords

  • 2A peptide
  • Carotenoid
  • Metabolic engineering
  • Polycistron
  • Rice

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biomedical Engineering

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