3,4-dideoxyglucosone-3-ene induces apoptosis in human peritoneal mesothelial cells

Duk Hyun Lee, Soon Youn Choi, Hye Myung Ryu, Chan Duck Kim, Sun Hee Park, Ho Young Chung, In-San Kim, Yong Lim Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

◆ Objective: Glucose degradation products (GDPs) are formed during heat sterilization and storage of peritoneal dialysis (PD) fluids. 3,4-dideoxyglucosone-3-ene (3,4-DGE) has been identified as the most bioreactive GDP. 3,4-DGE induces apoptosis in leukocytes and renal tubular epithelial cells. Our aim was to evaluate the apoptotic effects of 3,4-DGE on human peritoneal mesothelial cells (HPMCs). ◆ Methods: Primary cultured HPMCs were treated with 25 or 50 μmol/L3,4-DGE. MTT assay was used to determine cell viability. Apoptosis was measured using TUNEL assay and flow cytometry. Expressions of procaspase-3, Bax, and Bcl-2 were estimated by Western blot. Activity of caspase-3 was measured and the effect of the caspase inhibitor zVAD-fmk (Z-Val-Ala-DL-Asp-fluoromethylketone) was evaluated by TUNEL assay. ◆ Results: 3,4-DGE treatment accelerated cell death in HPMCs in a dose- and time-dependent manner. Treatment with 3,4-DGE (25 and 50 μmol/L) significantly increased apoptosis compared to control (p < 0.05 andp <0.01 respectively) by TUNEL assay. Flow cytometry showed treatment with 50 μmol/L 3,4-DGE significantly increased apoptosis compared to control (p < 0.05). Decreased expression of procaspase-3 and increased activity of caspase-3 were observed in the presence of 50 μmol/L 3,4-DGE compared to control and 25 μmol/L 3,4-DGE (p <0.05). 3,4-DGE-induced HPMC apoptosis was decreased after pretreatment with the pan-caspase inhibitor zVAD-fmk in the 50 μmol/L 3,4-DGE-treated group (p < 0.001). The ratio of Bcl-2 to Bax expression was decreased in the 25 μmol/-L and the 50 μmol/L 3,4-DGE-treated groups compared to control (p < 0.05). ◆ Conclusion: 3,4-DGE-promotes apoptosis in HPMCs by a caspase-related mechanism.

Original languageEnglish
Pages (from-to)44-51
Number of pages8
JournalPeritoneal Dialysis International
Volume29
Issue number1
Publication statusPublished - 2009 Jun 26
Externally publishedYes

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Apoptosis
Caspase 3
In Situ Nick-End Labeling
Caspase Inhibitors
3,4-dideoxyglucosone-3-ene
Flow Cytometry
Glucose
Ascitic Fluid
Peritoneal Dialysis
Caspases
Cell Survival
Leukocytes
Cell Death
Therapeutics
Hot Temperature
Western Blotting
Epithelial Cells
Kidney

Keywords

  • 3,4-DGE
  • Apoptosis
  • Caspase
  • Peritoneal mesothelial cells

ASJC Scopus subject areas

  • Nephrology

Cite this

Lee, D. H., Choi, S. Y., Ryu, H. M., Kim, C. D., Park, S. H., Chung, H. Y., ... Kim, Y. L. (2009). 3,4-dideoxyglucosone-3-ene induces apoptosis in human peritoneal mesothelial cells. Peritoneal Dialysis International, 29(1), 44-51.

3,4-dideoxyglucosone-3-ene induces apoptosis in human peritoneal mesothelial cells. / Lee, Duk Hyun; Choi, Soon Youn; Ryu, Hye Myung; Kim, Chan Duck; Park, Sun Hee; Chung, Ho Young; Kim, In-San; Kim, Yong Lim.

In: Peritoneal Dialysis International, Vol. 29, No. 1, 26.06.2009, p. 44-51.

Research output: Contribution to journalArticle

Lee, DH, Choi, SY, Ryu, HM, Kim, CD, Park, SH, Chung, HY, Kim, I-S & Kim, YL 2009, '3,4-dideoxyglucosone-3-ene induces apoptosis in human peritoneal mesothelial cells', Peritoneal Dialysis International, vol. 29, no. 1, pp. 44-51.
Lee DH, Choi SY, Ryu HM, Kim CD, Park SH, Chung HY et al. 3,4-dideoxyglucosone-3-ene induces apoptosis in human peritoneal mesothelial cells. Peritoneal Dialysis International. 2009 Jun 26;29(1):44-51.
Lee, Duk Hyun ; Choi, Soon Youn ; Ryu, Hye Myung ; Kim, Chan Duck ; Park, Sun Hee ; Chung, Ho Young ; Kim, In-San ; Kim, Yong Lim. / 3,4-dideoxyglucosone-3-ene induces apoptosis in human peritoneal mesothelial cells. In: Peritoneal Dialysis International. 2009 ; Vol. 29, No. 1. pp. 44-51.
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AU - Choi, Soon Youn

AU - Ryu, Hye Myung

AU - Kim, Chan Duck

AU - Park, Sun Hee

AU - Chung, Ho Young

AU - Kim, In-San

AU - Kim, Yong Lim

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N2 - ◆ Objective: Glucose degradation products (GDPs) are formed during heat sterilization and storage of peritoneal dialysis (PD) fluids. 3,4-dideoxyglucosone-3-ene (3,4-DGE) has been identified as the most bioreactive GDP. 3,4-DGE induces apoptosis in leukocytes and renal tubular epithelial cells. Our aim was to evaluate the apoptotic effects of 3,4-DGE on human peritoneal mesothelial cells (HPMCs). ◆ Methods: Primary cultured HPMCs were treated with 25 or 50 μmol/L3,4-DGE. MTT assay was used to determine cell viability. Apoptosis was measured using TUNEL assay and flow cytometry. Expressions of procaspase-3, Bax, and Bcl-2 were estimated by Western blot. Activity of caspase-3 was measured and the effect of the caspase inhibitor zVAD-fmk (Z-Val-Ala-DL-Asp-fluoromethylketone) was evaluated by TUNEL assay. ◆ Results: 3,4-DGE treatment accelerated cell death in HPMCs in a dose- and time-dependent manner. Treatment with 3,4-DGE (25 and 50 μmol/L) significantly increased apoptosis compared to control (p < 0.05 andp <0.01 respectively) by TUNEL assay. Flow cytometry showed treatment with 50 μmol/L 3,4-DGE significantly increased apoptosis compared to control (p < 0.05). Decreased expression of procaspase-3 and increased activity of caspase-3 were observed in the presence of 50 μmol/L 3,4-DGE compared to control and 25 μmol/L 3,4-DGE (p <0.05). 3,4-DGE-induced HPMC apoptosis was decreased after pretreatment with the pan-caspase inhibitor zVAD-fmk in the 50 μmol/L 3,4-DGE-treated group (p < 0.001). The ratio of Bcl-2 to Bax expression was decreased in the 25 μmol/-L and the 50 μmol/L 3,4-DGE-treated groups compared to control (p < 0.05). ◆ Conclusion: 3,4-DGE-promotes apoptosis in HPMCs by a caspase-related mechanism.

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