3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture

Wan Geun La, Suk Ho Bhang, Jung Youn Shin, Hee Hun Yoon, Jooyeon Park, Hee Seok Yang, Seung Ho Yu, Yung Eun Sung, Byung Soo Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.

Original languageEnglish
Pages (from-to)1055-1060
Number of pages6
JournalBiotechnology Progress
Volume28
Issue number4
DOIs
Publication statusPublished - 2012 Jul
Externally publishedYes

Keywords

  • 3,4-dihydroxy-L-phenylalanine
  • Cell adhesion
  • Cell adhesion molecule
  • Dermal fibroblasts
  • Serum-free culture

ASJC Scopus subject areas

  • Biotechnology

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