3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture

Wan Geun La, Suk Ho Bhang, Jung Youn Shin, Hee Hun Yoon, Jooyeon Park, Hee Seok Yang, Seung-Ho Yu, Yung Eun Sung, Byung Soo Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.

Original languageEnglish
Pages (from-to)1055-1060
Number of pages6
JournalBiotechnology Progress
Volume28
Issue number4
DOIs
Publication statusPublished - 2012 Jul 1
Externally publishedYes

Fingerprint

Cell Adhesion Molecules
Phenylalanine
Cell Culture Techniques
Serum
Fibroblasts
Photoelectron Spectroscopy
Skin
Serum-Free Culture Media
Fibronectins
Cell Adhesion
Spectrum Analysis

Keywords

  • 3,4-dihydroxy-L-phenylalanine
  • Cell adhesion
  • Cell adhesion molecule
  • Dermal fibroblasts
  • Serum-free culture

ASJC Scopus subject areas

  • Biotechnology

Cite this

La, W. G., Bhang, S. H., Shin, J. Y., Yoon, H. H., Park, J., Yang, H. S., ... Kim, B. S. (2012). 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture. Biotechnology Progress, 28(4), 1055-1060. https://doi.org/10.1002/btpr.1560

3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture. / La, Wan Geun; Bhang, Suk Ho; Shin, Jung Youn; Yoon, Hee Hun; Park, Jooyeon; Yang, Hee Seok; Yu, Seung-Ho; Sung, Yung Eun; Kim, Byung Soo.

In: Biotechnology Progress, Vol. 28, No. 4, 01.07.2012, p. 1055-1060.

Research output: Contribution to journalArticle

La, WG, Bhang, SH, Shin, JY, Yoon, HH, Park, J, Yang, HS, Yu, S-H, Sung, YE & Kim, BS 2012, '3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture', Biotechnology Progress, vol. 28, no. 4, pp. 1055-1060. https://doi.org/10.1002/btpr.1560
La, Wan Geun ; Bhang, Suk Ho ; Shin, Jung Youn ; Yoon, Hee Hun ; Park, Jooyeon ; Yang, Hee Seok ; Yu, Seung-Ho ; Sung, Yung Eun ; Kim, Byung Soo. / 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture. In: Biotechnology Progress. 2012 ; Vol. 28, No. 4. pp. 1055-1060.
@article{7b486accadba4b279c853710207ab854,
title = "3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture",
abstract = "In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.",
keywords = "3,4-dihydroxy-L-phenylalanine, Cell adhesion, Cell adhesion molecule, Dermal fibroblasts, Serum-free culture",
author = "La, {Wan Geun} and Bhang, {Suk Ho} and Shin, {Jung Youn} and Yoon, {Hee Hun} and Jooyeon Park and Yang, {Hee Seok} and Seung-Ho Yu and Sung, {Yung Eun} and Kim, {Byung Soo}",
year = "2012",
month = "7",
day = "1",
doi = "10.1002/btpr.1560",
language = "English",
volume = "28",
pages = "1055--1060",
journal = "Biotechnology Progress",
issn = "8756-7938",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

TY - JOUR

T1 - 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture

AU - La, Wan Geun

AU - Bhang, Suk Ho

AU - Shin, Jung Youn

AU - Yoon, Hee Hun

AU - Park, Jooyeon

AU - Yang, Hee Seok

AU - Yu, Seung-Ho

AU - Sung, Yung Eun

AU - Kim, Byung Soo

PY - 2012/7/1

Y1 - 2012/7/1

N2 - In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.

AB - In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.

KW - 3,4-dihydroxy-L-phenylalanine

KW - Cell adhesion

KW - Cell adhesion molecule

KW - Dermal fibroblasts

KW - Serum-free culture

UR - http://www.scopus.com/inward/record.url?scp=84864838061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864838061&partnerID=8YFLogxK

U2 - 10.1002/btpr.1560

DO - 10.1002/btpr.1560

M3 - Article

VL - 28

SP - 1055

EP - 1060

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 8756-7938

IS - 4

ER -