3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture

Wan Geun La, Suk Ho Bhang, Jung Youn Shin, Hee Hun Yoon, Jooyeon Park, Hee Seok Yang, Seung-Ho Yu, Yung Eun Sung, Byung Soo Kim

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Abstract

In this article, we examined the feasibility of using 3,4-dihydroxy-L-phenylalanine (DOPA) as a cell adhesion molecule in serum-free cultures of anchorage-dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X-ray photoelectron spectroscopy and energy-dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA-coated, fibronectin-coated, or no material-coated culture plates in serum-free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell-adhesion molecule for serum-free culture.

Original languageEnglish
Pages (from-to)1055-1060
Number of pages6
JournalBiotechnology Progress
Volume28
Issue number4
DOIs
Publication statusPublished - 2012 Jul 1
Externally publishedYes

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Keywords

  • 3,4-dihydroxy-L-phenylalanine
  • Cell adhesion
  • Cell adhesion molecule
  • Dermal fibroblasts
  • Serum-free culture

ASJC Scopus subject areas

  • Biotechnology

Cite this

La, W. G., Bhang, S. H., Shin, J. Y., Yoon, H. H., Park, J., Yang, H. S., Yu, S-H., Sung, Y. E., & Kim, B. S. (2012). 3,4-dihydroxy-L-phenylalanine as a cell adhesion molecule in serum-free cell culture. Biotechnology Progress, 28(4), 1055-1060. https://doi.org/10.1002/btpr.1560