3D-cultured human placenta-derived mesenchymal stem cell spheroids enhance ovary function by inducing folliculogenesis

Tae Hee Kim, Jong Ho Choi, Yesl Jun, Seung Mook Lim, Sohae Park, Jin Young Paek, Sang Hoon Lee, Ji Young Hwang, Gi Jin Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Placenta-derived mesenchymal stem cells (PD-MSCs) have numerous advantages over other adult MSCs that make them an attractive cell source for regenerative medicine. Here, we demonstrate the therapeutic effect of PD-MSCs in ovariectomized (Ovx) rats and compare their efficacy when generated via a conventional monolayer culture system (2D, naïve) and a spheroid culture system (3D, spheroid). PD-MSC transplantation significantly increased the estradiol level in Ovx rats compared with the non-transplantation (NTx) group. In particular, the estradiol level in the Spheroid group was significantly higher than that in the Naïve group at 2 weeks. Spheroid PD-MSCs exhibited a significantly higher efficiency of engraftment onto ovarian tissues at 2 weeks. The mRNA and protein expression levels of Nanos3, Nobox, and Lhx8 were also significantly increased in the Spheroid group compared with those in the NTx group at 1 and 2 weeks. These results suggest that PD-MSC transplantation can restore ovarian function in Ovx rats by increasing estrogen production and enhancing folliculogenesis-related gene expression levels and further indicate that spheroid-cultured PD-MSCs have enhanced therapeutic potential via increased engraftment efficiency. These findings improve our understanding of stem-cell-based therapies for reproductive systems and may suggest new avenues for developing efficient therapies using 3D cultivation systems.

Original languageEnglish
Article number15313
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Mesenchymal Stromal Cells
Placenta
Ovary
Mesenchymal Stem Cell Transplantation
Estradiol
Regenerative Medicine
Therapeutic Uses
Cell- and Tissue-Based Therapy
Estrogens
Stem Cells
Gene Expression
Messenger RNA
Therapeutics
Proteins

ASJC Scopus subject areas

  • General

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3D-cultured human placenta-derived mesenchymal stem cell spheroids enhance ovary function by inducing folliculogenesis. / Kim, Tae Hee; Choi, Jong Ho; Jun, Yesl; Lim, Seung Mook; Park, Sohae; Paek, Jin Young; Lee, Sang Hoon; Hwang, Ji Young; Kim, Gi Jin.

In: Scientific Reports, Vol. 8, No. 1, 15313, 01.12.2018.

Research output: Contribution to journalArticle

Kim, Tae Hee ; Choi, Jong Ho ; Jun, Yesl ; Lim, Seung Mook ; Park, Sohae ; Paek, Jin Young ; Lee, Sang Hoon ; Hwang, Ji Young ; Kim, Gi Jin. / 3D-cultured human placenta-derived mesenchymal stem cell spheroids enhance ovary function by inducing folliculogenesis. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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