3D printed alendronate-releasing poly(caprolactone) porous scaffolds enhance osteogenic differentiation and bone formation in rat tibial defects

Sung Eun Kim, Young Pil Yun, Kyu Sik Shim, Hak Jun Kim, Kyeongsoon Park, Hae Ryong Song

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14 Citations (Scopus)


The aim of this study was to evaluate the in vitro osteogenic effects and in vivo new bone formation of three-dimensional (3D) printed alendronate (Aln)-releasing poly(caprolactone) (PCL) (Aln/PCL) scaffolds in rat tibial defect models. 3D printed Aln/PCL scaffolds were fabricated via layer-by-layer deposition. The fabricated Aln/PCL scaffolds had high porosity and an interconnected pore structure and showed sustained Aln release. In vitro studies showed that MG-63 cells seeded on the Aln/PCL scaffolds displayed increased alkaline phosphatase (ALP) activity and calcium content in a dose-dependent manner when compared with cell cultures in PCL scaffolds. In addition, in vivo animal studies and histologic evaluation showed that Aln/PCL scaffolds implanted in a rat tibial defect model markedly increased new bone formation and mineralized bone tissues in a dose-dependent manner compared to PCL-only scaffolds. Our results show that 3D printed Aln/PCL scaffolds are promising templates for bone tissue engineering applications.

Original languageEnglish
Article number055005
JournalBiomedical Materials (Bristol)
Issue number5
Publication statusPublished - 2016 Sep 29



  • alendronate
  • bone formation
  • MG-63 cells
  • three-dimensional (3D) printed scaffold
  • tibial defect model

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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