4-Hydroxybenzaldehyde sensitizes Acinetobacter baumannii to amphenicols

Bora Shin, Chulwoo Park, James A. Imlay, Woojun Park

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Bacterial metabolism modulated by environmental chemicals could alter antibiotic susceptibility. 4-Hydroxybenzaldehyde (4-HBA), which cannot support the growth of Acinetobacter baumannii, exhibited synergism only with amphenicol antibiotics including chloramphenicol (CAM) and thiamphenicol. Interestingly, this synergistic effect was not observed with other growth-supporting, structurally similar compounds such as 4-hydroxybenzoate. Transcriptomic analysis demonstrated that genes involved in protocatechuate metabolism (pca genes) and osmotic stress (bet genes) were significantly upregulated by 4-HBA and CAM treatment. The 14C-labeled CAM influx was lower in a pcaK1 (encoding a transporter of protocatechuate) deletion mutant and was higher in the pcaK1 overexpressing cells relative to that in the wild type upon 4-HBA treatment. Our kinetic data using 14C-labeled CAM clearly showed that CAM uptake is possibly through facilitated diffusion. Deletion of pcaK1 did not result in the elimination of CAM influx, indicating that CAM does not enter only through PcaK1. The amount of 4-HBA in the culture supernatant was, however, unaffected during the test conditions, validating that it was not metabolized by the bacteria. CAM resistant A. baumannii cells derived by serial passages through CAM-amended media exhibited lower level of pcaK1 gene expression. These results led us to conclude that the activation of PcaK1 transporter is probably linked to cellular CAM susceptibility. This is the first report showing a relationship between CAM influx and aromatic compound metabolism in A. baumannii.

Original languageEnglish
Pages (from-to)2323-2335
Number of pages13
JournalApplied Microbiology and Biotechnology
Issue number5
Publication statusPublished - 2018 Mar 1


  • 4-Hydroxybenzaldehyde
  • Acinetobacter
  • Chloramphenicol
  • Phenolic compound
  • Plant extract
  • Synergism
  • Synergistic compound

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


Dive into the research topics of '4-Hydroxybenzaldehyde sensitizes Acinetobacter baumannii to amphenicols'. Together they form a unique fingerprint.

Cite this