4,4'-Dichlorodiphenyltrichloroethane (DDT) and 4,4'-dichlorodiphenyldichloroethylene (DDE) promote adipogenesis in 3T3-L1 adipocyte cell culture

Jonggun Kim, Quancai Sun, Yiren Yue, Kyong Sup Yoon, Kwang Youn Whang, J. Marshall Clark, Yeonhwa Park

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

4,4'-Dichlorodiphenyltrichloroethane (DDT), a chlorinated hydrocarbon insecticide, was extensively used in the 1940s and 1950s. DDT is mainly metabolically converted into 4,4'-dichlorodiphenyldichloroethylene (DDE). Even though most countries banned DDT in the 1970s, due to the highly lipophilic nature and very stable characteristics, DDT and its metabolites are present ubiquitously in the environment, including food. Recently, there are publications on relationships between exposure to insecticides, including DDT and DDE, and weight gain and altered glucose homeostasis. However, there are limited reports regarding DDT or DDE and adipogenesis, thus we investigated effects of DDT and DDE on adipogenesis using 3T3-L1 adipocytes. Treatment of DDT or DDE resulted in increased lipid accumulation accompanied by increased expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome-proliferator activated receptor-γ (PPARγ), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), adipose triglyceride lipase, and leptin. Moreover, treatment of DDT or DDE increased protein levels of C/EBPα, PPARγ, AMP-activated protein kinase-α (AMPKα), and ACC, while significant decrease of phosphorylated forms of AMPKα and ACC were observed. These finding suggest that increased lipid accumulation caused by DDT and DDE may mediate AMPKα pathway in 3T3-L1 adipocytes.

Original languageEnglish
Pages (from-to)40-45
Number of pages6
JournalPesticide Biochemistry and Physiology
Volume131
DOIs
Publication statusPublished - 2016 Jul 1

Fingerprint

3T3-L1 Cells
Acetyl-CoA Carboxylase
Adipogenesis
AMP-Activated Protein Kinases
DDT
DDT (pesticide)
adipocytes
Adipocytes
CCAAT-Enhancer-Binding Proteins
Peroxisome Proliferator-Activated Receptors
cell culture
Cell Culture Techniques
Insecticides
Chlorinated Hydrocarbons
Lipids
Fatty Acid Synthases
AMP-activated protein kinase
acetyl-CoA carboxylase
Leptin
Lipase

Keywords

  • 3T3-L1
  • Adipocyte differentiation
  • Adipogenesis
  • DDE
  • DDT
  • Lipogenesis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

4,4'-Dichlorodiphenyltrichloroethane (DDT) and 4,4'-dichlorodiphenyldichloroethylene (DDE) promote adipogenesis in 3T3-L1 adipocyte cell culture. / Kim, Jonggun; Sun, Quancai; Yue, Yiren; Yoon, Kyong Sup; Whang, Kwang Youn; Marshall Clark, J.; Park, Yeonhwa.

In: Pesticide Biochemistry and Physiology, Vol. 131, 01.07.2016, p. 40-45.

Research output: Contribution to journalArticle

Kim, Jonggun ; Sun, Quancai ; Yue, Yiren ; Yoon, Kyong Sup ; Whang, Kwang Youn ; Marshall Clark, J. ; Park, Yeonhwa. / 4,4'-Dichlorodiphenyltrichloroethane (DDT) and 4,4'-dichlorodiphenyldichloroethylene (DDE) promote adipogenesis in 3T3-L1 adipocyte cell culture. In: Pesticide Biochemistry and Physiology. 2016 ; Vol. 131. pp. 40-45.
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abstract = "4,4'-Dichlorodiphenyltrichloroethane (DDT), a chlorinated hydrocarbon insecticide, was extensively used in the 1940s and 1950s. DDT is mainly metabolically converted into 4,4'-dichlorodiphenyldichloroethylene (DDE). Even though most countries banned DDT in the 1970s, due to the highly lipophilic nature and very stable characteristics, DDT and its metabolites are present ubiquitously in the environment, including food. Recently, there are publications on relationships between exposure to insecticides, including DDT and DDE, and weight gain and altered glucose homeostasis. However, there are limited reports regarding DDT or DDE and adipogenesis, thus we investigated effects of DDT and DDE on adipogenesis using 3T3-L1 adipocytes. Treatment of DDT or DDE resulted in increased lipid accumulation accompanied by increased expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome-proliferator activated receptor-γ (PPARγ), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), adipose triglyceride lipase, and leptin. Moreover, treatment of DDT or DDE increased protein levels of C/EBPα, PPARγ, AMP-activated protein kinase-α (AMPKα), and ACC, while significant decrease of phosphorylated forms of AMPKα and ACC were observed. These finding suggest that increased lipid accumulation caused by DDT and DDE may mediate AMPKα pathway in 3T3-L1 adipocytes.",
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AU - Sun, Quancai

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