7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates β-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus

Chakradhar Dasagrandhi, Young Soon Kim, In Hwan Kim, Ching T. Hou, Hak Ryul Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infections with multi-drug resistance needs effective and alternative control strategies. In this study we investigated the adjuvant effect of a novel furan fatty acid, 7,10-epoxyoctadeca-7,9-dienoic acid (7,10-EODA) against multidrug-resistant S. aureus (MDRSA) strain 01ST001 by disc diffusion, checker board and time kill assays. Further the membrane targeting action of 7,10-EODA was investigated by spectroscopic and confocal microscopic studies. 7,10-EODA exerted synergistic activity along with β-lactam antibiotics against all clinical MRSA strains, with a mean fractional inhibitory concentration index below 0.5. In time-kill kinetic study, combination of 7,10-EODA with oxacillin, ampicillin, and penicillin resulted in 3.8–4.2 log10 reduction in the viable counts of MDRSA 01ST001. Further, 7,10-EODA dose dependently altered the membrane integrity (p < 0.001) and increased the binding of fluorescent analog of penicillin, Bocillin-FL to the MDRSA cells. The membrane action of 7,10-EODA further facilitated the uptake of several other antibiotics in MDRSA. The results of the present study suggested that 7,10-EODA could be a novel antibiotic adjuvant, especially useful in repurposing β-lactam antibiotics against multidrug-resistant MRSA.

Original languageEnglish
Pages (from-to)461-469
Number of pages9
JournalIndian Journal of Microbiology
Volume57
Issue number4
DOIs
Publication statusPublished - 2017 Dec 1

Keywords

  • 7,10-Epoxyoctadeca-7,9-dienoic acid
  • Furan fatty acids
  • Multidrug-resistant Staphylococcus aureus
  • Synergistic antibacterial agent
  • β-Lactam antibiotics

ASJC Scopus subject areas

  • Microbiology

Fingerprint Dive into the research topics of '7,10-Epoxyoctadeca-7,9-dienoic Acid: A Small Molecule Adjuvant That Potentiates β-Lactam Antibiotics Against Multidrug-Resistant Staphylococcus aureus'. Together they form a unique fingerprint.

  • Cite this