A Bacterial mRNA Leader that Employs Different Mechanisms to Sense Disparate Intracellular Signals

Sun Yang Park, Michael J. Cromie, Eun-Jin Lee, Eduardo A. Groisman

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Bacterial mRNAs often contain leader sequences that respond to specific metabolites or ions by altering expression of the associated downstream protein-coding sequences. Here we report that the leader RNA of the Mg2+ transporter gene mgtA of Salmonella enterica, which was previously known to function as a Mg2+-sensing riboswitch, harbors an 18 codon proline-rich open reading frame-termed mgtL-that permits intracellular proline to regulate mgtA expression. Interfering with mgtL translation by genetic, pharmacological, or environmental means was observed to increase the mRNA levels from the mgtA coding region. Substitution of the mgtL proline codons by other codons abolished the response to proline and to hyperosmotic stress but not to Mg2+. Our findings show that mRNA leader sequences can consist of complex regulatory elements that utilize different mechanisms to sense separate signals and mediate an appropriate cellular response.

Original languageEnglish
Pages (from-to)737-748
Number of pages12
JournalCell
Volume142
Issue number5
DOIs
Publication statusPublished - 2010 Sep 3
Externally publishedYes

Fingerprint

Proline
Codon
Messenger RNA
Riboswitch
Salmonella
Salmonella enterica
5' Untranslated Regions
Protein Biosynthesis
Metabolites
Ports and harbors
Open Reading Frames
Substitution reactions
Genes
Pharmacology
RNA
Ions
Proteins

Keywords

  • Microbio
  • RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A Bacterial mRNA Leader that Employs Different Mechanisms to Sense Disparate Intracellular Signals. / Park, Sun Yang; Cromie, Michael J.; Lee, Eun-Jin; Groisman, Eduardo A.

In: Cell, Vol. 142, No. 5, 03.09.2010, p. 737-748.

Research output: Contribution to journalArticle

Park, Sun Yang ; Cromie, Michael J. ; Lee, Eun-Jin ; Groisman, Eduardo A. / A Bacterial mRNA Leader that Employs Different Mechanisms to Sense Disparate Intracellular Signals. In: Cell. 2010 ; Vol. 142, No. 5. pp. 737-748.
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