A biosensor for the detection of gas toxicity using a recombinant bioluminescent bacterium

Geun Cheol Gil, Robert J. Mitchell, Suk Tai Chang, Man Bock Gu

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82 Citations (Scopus)

Abstract

A whole-cell biosensor was developed for the detection of gas toxicity using a recombinant bioluminescent Escherichia coli harboring a lac::luxCDABE fusion. Immobilization of the cells within LB agar has been done to maintain the activity of the microorganisms and to detect the toxicity of chemicals through the direct contact with gas. Benzene, known as a representative volatile organic compound, was chosen as a sample toxic gas to evaluate the performance of this biosensor based on the bioluminescent response. This biosensor showed a dose-dependent response, and was found to be reproducible. The immobilizing matrices of this biosensor were stored at 4°C and were maintained for at least a month without any noticible change in its activity. The optimal temperature for sensing was 37°C. A small size of this sensor kit has been successfully fabricated, and found to be applicable as a disposable and portable biosensor to monitor the atmospheric environment of a workplace in which high concentrations of toxic gases could be discharged.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalBiosensors and Bioelectronics
Volume15
Issue number1-2
DOIs
Publication statusPublished - 2000 Dec 1
Externally publishedYes

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Keywords

  • Benzene
  • Gas toxicity
  • Recombinant bioluminescent Escherichia coli
  • Whole cell biosensor

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

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