A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells

Kondapa Naidu Bobba, Miae Won, Inseob Shim, Nithya Velusamy, Zhigang Yang, Junle Qu, Jong Seung Kim, Sankarprasad Bhuniya

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18 Citations (Scopus)

Abstract

Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λem = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about H2O2-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.

Original languageEnglish
Pages (from-to)11213-11216
Number of pages4
JournalChemical Communications
Volume53
Issue number81
DOIs
Publication statusPublished - 2017

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ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Naidu Bobba, K., Won, M., Shim, I., Velusamy, N., Yang, Z., Qu, J., Kim, J. S., & Bhuniya, S. (2017). A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells. Chemical Communications, 53(81), 11213-11216. https://doi.org/10.1039/c7cc05043g