TY - JOUR
T1 - A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells
AU - Naidu Bobba, Kondapa
AU - Won, Miae
AU - Shim, Inseob
AU - Velusamy, Nithya
AU - Yang, Zhigang
AU - Qu, Junle
AU - Kim, Jong Seung
AU - Bhuniya, Sankarprasad
N1 - Funding Information:
This work was supported by CRI (No. 2009-0081566, JSK) project grants and NRF2010-0020209 from the National Research Foundation of Korea. This work was also supported by DST-SERB, India, through a research grant (No. ECR/2015/00035, SB). In addition, this work was supported by the National Natural Science Foundation of China (NSFC) (No. 21406125/61525503/ 61378091/61620106016) and the National Basic Research Program of China (No. 2015CB352005).
Publisher Copyright:
© 2017 The Royal Society of Chemistry.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2017
Y1 - 2017
N2 - Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λem = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about H2O2-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.
AB - Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λem = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about H2O2-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.
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U2 - 10.1039/c7cc05043g
DO - 10.1039/c7cc05043g
M3 - Article
C2 - 28956555
AN - SCOPUS:85031003246
SN - 1359-7345
VL - 53
SP - 11213
EP - 11216
JO - Chemical Communications
JF - Chemical Communications
IS - 81
ER -