A cell array biosensor for environmental toxicity analysis

Hyung Lee Jin, Robert J. Mitchell, Chan Kim Byoung, David C. Cullen, Bock Gu Man

Research output: Contribution to journalArticlepeer-review

108 Citations (Scopus)

Abstract

In this study, a cell-based array technology that uses recombinant bioluminescent bacteria to detect and classify environmental toxicity has been implemented to develop two biosensor arrays, i.e., a chip and a plate array. Twenty recombinant bioluminescent bacteria, having different promoters fused with the bacterial lux genes, were immobilized within LB-agar. About 2 μl of the cell-agar mixture was deposited into the wells of either a cell chip or a 384-well plate. The bioluminescence (BL) from the cell arrays was measured with the use of highly sensitive cooled CCD camera that measured the bioluminescent signal from the immobilized cells and then quantified the pixel density using image analysis software. The responses from the cell arrays were characterized using three chemicals that cause either superoxide damage (paraquat), DNA damage (mitomycin C) or protein/membrane damage (salicylic acid). The responses were found to be dependent upon the promoter fused upstream of the lux operon within each strain. Therefore, a sample's toxicity can be analyzed and classified through the changes in the BL expression from each well. Moreover, a time of only 2 h was needed for analysis, making either of these arrays a fast, portable and economical high-throughput biosensor system for detecting environmental toxicities.

Original languageEnglish
Pages (from-to)500-507
Number of pages8
JournalBiosensors and Bioelectronics
Volume21
Issue number3
DOIs
Publication statusPublished - 2005 Sep 15
Externally publishedYes

Keywords

  • Bioluminescent bacteria
  • Cell array chip
  • Environmental biosensor
  • Toxicity analysis

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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