A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase e has synergistic effects on galactomannan degradation

Sang Duck Jeon, Kyung Ok Yu, Seung Wook Kim, Sung Ok Han

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In our previous study using a fluorescently labeled cohesin biomarker, we detected and identified a putative cellulosomal mannanase belonging to the glycosyl hydrolase family 26 from Clostridium cellulovorans in xylan-containing cultures. In this study, a mannanase gene, manB from C. cellulovorans, was expressed in Escherichia coli. The optimal pH of a purified enzyme was around pH 7.0 and the optimal temperature was 40°C. The purified mannanase B (ManB) showed high hydrolytic activity toward galactomannan. An assembly of ManB with mini-CbpA, which contains a carbohydrate-binding module that provides proximity to insoluble substrates, increased the activity toward galactomannan [locust bean gum (LBG) and guar gum] 1.7- and 2.0-fold over those without mini-CbpA. We tested the synergistic effects on galactomannan (LBG and guar gum) degradation using cellulosomal mannanase ManB with cellulosomal endoglucanase E, which was predicted to have mannanase activity in C. cellulovorans as a cellulolytic complex. When assembled with the mini-CbpA, the mixture of endoglucanase E (EngE) and ManB at a molar ratio of 1:2 showed the highest synergistic effect (2.4-fold) on LBG. The mixture at a ratio of 1:3 showed the highest synergistic effect (2.8-fold) on guar gum. These synergistic actions indicated that ManB assembled with mini-CbpA hydrolyzed insoluble galactomannan, which in turn promoted soluble galactomannan degradation by EngE.

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume90
Issue number2
DOIs
Publication statusPublished - 2011 Apr

Keywords

  • Cellulosome
  • Clostridium cellulovorans
  • Galactomannan
  • Mannanase
  • Synergistic effect

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'A celluloytic complex from Clostridium cellulovorans consisting of mannanase B and endoglucanase e has synergistic effects on galactomannan degradation'. Together they form a unique fingerprint.

  • Cite this