A comparative study of analytical methods for alkali-soluble β-glucan in medicinal mushroom, Chaga (Inonotus obliquus)

Sook Jong Rhee; Seung Yong Cho; Ki Myong Kim; Dong Su Cha; Hyun Jin Park

doi:10.1016/j.lwt.2007.03.028

A comparative study of analytical methods for alkali-soluble β-glucan in medicinal mushroom, Chaga (Inonotus obliquus)

Sook Jong Rhee, Seung Yong Cho, Ki Myong Kim, Dong Su Cha, Hyun Jin Park

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Crude β-glucan content in the medicinal mushroom Chaga (Inonotus obliquus) was measured by different extraction and analytical methods, and the results were compared. The alkali extraction (AE) method or enzymatic digestion (ED) method followed by a gravimetric analysis was employed to determine the crude β-glucan content. The amount of crude β-glucan in Chaga obtained by either AE or ED was 13.7 g/100 g or 15.3 g/100 g of the sample, respectively. Crude β-glucan content of Chaga obtained by the above preparation methods were corrected by chemical composition analysis and HPLC analysis. After composition analysis, the amounts of β-glucan measured in the 100 g Chaga samples were corrected to 10.1 g for ED and 10.7 g for AE method. β-glucan contents calculated by the amount of glucose in the HPLC analysis were 8.3 g/100 g and 8.1 g/100 g for ED and AE preparation methods, respectively. Although extraction method did not affect β-glucan content in Chaga as indicated by no significant difference (P>0.05) between extraction method, significant differences (P<0.05) were noted between the correction methods. The discrepancies of the result indicate a need for standardization of analytical method for β-glucan measurement in Chaga.

Original language	English
Pages (from-to)	545-549
Number of pages	5
Journal	LWT
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/j.lwt.2007.03.028
Publication status	Published - 2008 Apr

Keywords

Analytical method
Chaga
Inonotus obliquus
Medicinal mushroom
Standardization
β-glucan

ASJC Scopus subject areas

Food Science

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10.1016/j.lwt.2007.03.028

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title = "A comparative study of analytical methods for alkali-soluble β-glucan in medicinal mushroom, Chaga (Inonotus obliquus)",

abstract = "Crude β-glucan content in the medicinal mushroom Chaga (Inonotus obliquus) was measured by different extraction and analytical methods, and the results were compared. The alkali extraction (AE) method or enzymatic digestion (ED) method followed by a gravimetric analysis was employed to determine the crude β-glucan content. The amount of crude β-glucan in Chaga obtained by either AE or ED was 13.7 g/100 g or 15.3 g/100 g of the sample, respectively. Crude β-glucan content of Chaga obtained by the above preparation methods were corrected by chemical composition analysis and HPLC analysis. After composition analysis, the amounts of β-glucan measured in the 100 g Chaga samples were corrected to 10.1 g for ED and 10.7 g for AE method. β-glucan contents calculated by the amount of glucose in the HPLC analysis were 8.3 g/100 g and 8.1 g/100 g for ED and AE preparation methods, respectively. Although extraction method did not affect β-glucan content in Chaga as indicated by no significant difference (P>0.05) between extraction method, significant differences (P<0.05) were noted between the correction methods. The discrepancies of the result indicate a need for standardization of analytical method for β-glucan measurement in Chaga.",

keywords = "Analytical method, Chaga, Inonotus obliquus, Medicinal mushroom, Standardization, β-glucan",

author = "Rhee, {Sook Jong} and Cho, {Seung Yong} and Kim, {Ki Myong} and Cha, {Dong Su} and Park, {Hyun Jin}",

note = "Funding Information: This work was supported by a grant (07062KFDA104) from Korea Food & Drug Administration in 2007, and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2004-216-F00232). ",

year = "2008",

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doi = "10.1016/j.lwt.2007.03.028",

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T1 - A comparative study of analytical methods for alkali-soluble β-glucan in medicinal mushroom, Chaga (Inonotus obliquus)

AU - Rhee, Sook Jong

AU - Cho, Seung Yong

AU - Kim, Ki Myong

AU - Cha, Dong Su

AU - Park, Hyun Jin

N1 - Funding Information: This work was supported by a grant (07062KFDA104) from Korea Food & Drug Administration in 2007, and by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2004-216-F00232).

PY - 2008/4

Y1 - 2008/4

N2 - Crude β-glucan content in the medicinal mushroom Chaga (Inonotus obliquus) was measured by different extraction and analytical methods, and the results were compared. The alkali extraction (AE) method or enzymatic digestion (ED) method followed by a gravimetric analysis was employed to determine the crude β-glucan content. The amount of crude β-glucan in Chaga obtained by either AE or ED was 13.7 g/100 g or 15.3 g/100 g of the sample, respectively. Crude β-glucan content of Chaga obtained by the above preparation methods were corrected by chemical composition analysis and HPLC analysis. After composition analysis, the amounts of β-glucan measured in the 100 g Chaga samples were corrected to 10.1 g for ED and 10.7 g for AE method. β-glucan contents calculated by the amount of glucose in the HPLC analysis were 8.3 g/100 g and 8.1 g/100 g for ED and AE preparation methods, respectively. Although extraction method did not affect β-glucan content in Chaga as indicated by no significant difference (P>0.05) between extraction method, significant differences (P<0.05) were noted between the correction methods. The discrepancies of the result indicate a need for standardization of analytical method for β-glucan measurement in Chaga.

AB - Crude β-glucan content in the medicinal mushroom Chaga (Inonotus obliquus) was measured by different extraction and analytical methods, and the results were compared. The alkali extraction (AE) method or enzymatic digestion (ED) method followed by a gravimetric analysis was employed to determine the crude β-glucan content. The amount of crude β-glucan in Chaga obtained by either AE or ED was 13.7 g/100 g or 15.3 g/100 g of the sample, respectively. Crude β-glucan content of Chaga obtained by the above preparation methods were corrected by chemical composition analysis and HPLC analysis. After composition analysis, the amounts of β-glucan measured in the 100 g Chaga samples were corrected to 10.1 g for ED and 10.7 g for AE method. β-glucan contents calculated by the amount of glucose in the HPLC analysis were 8.3 g/100 g and 8.1 g/100 g for ED and AE preparation methods, respectively. Although extraction method did not affect β-glucan content in Chaga as indicated by no significant difference (P>0.05) between extraction method, significant differences (P<0.05) were noted between the correction methods. The discrepancies of the result indicate a need for standardization of analytical method for β-glucan measurement in Chaga.

KW - Analytical method

KW - Chaga

KW - Inonotus obliquus

KW - Medicinal mushroom

KW - Standardization

KW - β-glucan

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JO - LWT - Food Science and Technology

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A comparative study of analytical methods for alkali-soluble β-glucan in medicinal mushroom, Chaga (Inonotus obliquus)

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