A Comparison of Embryonic Development and Clinical Outcomes between in vitro Oocytes Maturation Using Micro-Vibration System and in vivo Oocytes Maturation in Polycystic Ovarian Syndrome Patients

Seong Ho Yang, Yong Soo Hur, San Hyun Yoon, Jae Hoon Jung, Jin Ho Lim, Yong Ko

Research output: Contribution to journalArticle


Background/Objectives: Mechanical micro-vibration remains insufficient for improving embryo culture conditions in human immature oocytes. This study compared the clinical outcomes and embryo development between germinal vesicle (GV) oocytes with the micro-vibration culture (MVC) system in in vitro maturation (IVM) cycles and in vivo-matured oocytes in controlled ovarian hyperstimulation (COH) cycles in polycystic ovarian syndrome (PCOS) patients. Methods: This study investigated 152 PCOS patients who underwent 159 fresh embryo transfer cycles, including IVM cycles with embryos derived from GV oocytes and the COH cycles with embryos derived from in vivo-matured oocytes. The IVM cycles were divided into groups according to the culture system used: static culture (SC) and MVC: In the IVM-S group (n = 47), SC was applied during both IVM and in vitro culture (IVC), whereas in the IVM-MV group (n = 44), MVC was applied during both IVM and IVC. For the COH cycles, in the COH-S group (n = 68), SC was applied during IVC. Results: The number of in vitro-matured oocytes was similar in the IVM-S and IVM-MV groups, but the good-quality embryo (GQE; ≥6-cells) rate was significantly higher in the IVM-MV group (p < 0.01). The GQE rate and clinical outcomes of the COH-S group were significantly better than those of the IVM-S group (p < 0.05) but similar to those of the IVM-MV group. Conclusion: Compared with the SC system, the MVC system in IVM cycles improves the embryonic quality of GV oocytes and clinical outcomes, resulting in development of potential equivalent to in vivo-matured oocytes.

Original languageEnglish
JournalGynecologic and Obstetric Investigation
Publication statusAccepted/In press - 2020 Jan 1


  • Germinal vesicle oocytes
  • In vitro maturation
  • In vivo maturation
  • Micro-vibration culture system
  • Polycystic ovarian syndrome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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