A comprehensive karyotypic analysis on Korean hepatocellular carcinoma cell lines by cross-species color banding and comparative genomic hybridization

Hye Jin Hwang, Gi Jin Kim, Gi Bong Lee, Jung Tak Oh, Yong Hyuck Chun, Sun Hwa Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chromosomal aberrations were investigated in hepatitis B virus integrated into the hepatocellular carcinoma (HCC) cell lines SNU-368, SNU-449, SNU-398, SNU-182, and SNU-475 using Giemsa-banding, cross species color banding, and comparative genomic hybridization (CGH). The origins of the marker chromosomes were confirmed by fluorescence in situ hybridization with constructed chromosome painting probes. Each cell line had unique modal karyotypic characteristics and showed variable numbers of numerical and structural clonal cytogenetic aberrations. The gains were commonly detected on chromosome 1, and chromosome regions 6p, 7q, 8q, 10p, 17q, and 20; the losses were often found on 4q21∼qter, 13, 18q21∼qter, and Y. In particular, the breakpoints on 1p36, 1p13∼q21, 2p13∼q11, 6q10∼q11, 7q11, 7q22, 14q10, 16q10∼q13, 17q21, 18q21, and 19p11∼q11 were involved frequently at the multiple rearranged lesions. CGH analysis further confirmed the cytogenetic data, and the nonrandom rearrangements data suggested the candidate regions for the genes to be isolated which were related to HCC.

Original languageEnglish
Pages (from-to)128-137
Number of pages10
JournalCancer Genetics and Cytogenetics
Volume141
Issue number2
DOIs
Publication statusPublished - 2003 Mar

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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