Abstract
Chromosomal aberrations were investigated in hepatitis B virus integrated into the hepatocellular carcinoma (HCC) cell lines SNU-368, SNU-449, SNU-398, SNU-182, and SNU-475 using Giemsa-banding, cross species color banding, and comparative genomic hybridization (CGH). The origins of the marker chromosomes were confirmed by fluorescence in situ hybridization with constructed chromosome painting probes. Each cell line had unique modal karyotypic characteristics and showed variable numbers of numerical and structural clonal cytogenetic aberrations. The gains were commonly detected on chromosome 1, and chromosome regions 6p, 7q, 8q, 10p, 17q, and 20; the losses were often found on 4q21∼qter, 13, 18q21∼qter, and Y. In particular, the breakpoints on 1p36, 1p13∼q21, 2p13∼q11, 6q10∼q11, 7q11, 7q22, 14q10, 16q10∼q13, 17q21, 18q21, and 19p11∼q11 were involved frequently at the multiple rearranged lesions. CGH analysis further confirmed the cytogenetic data, and the nonrandom rearrangements data suggested the candidate regions for the genes to be isolated which were related to HCC.
| Original language | English |
|---|---|
| Pages (from-to) | 128-137 |
| Number of pages | 10 |
| Journal | Cancer Genetics and Cytogenetics |
| Volume | 141 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 Mar 1 |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cancer Research
Cite this
A comprehensive karyotypic analysis on Korean hepatocellular carcinoma cell lines by cross-species color banding and comparative genomic hybridization. / Hwang, Hye Jin; Kim, Gi Jin; Lee, Gi Bong; Oh, Jung Tak; Chun, Yong Hyuck; Park, Sun-Hwa.
In: Cancer Genetics and Cytogenetics, Vol. 141, No. 2, 01.03.2003, p. 128-137.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A comprehensive karyotypic analysis on Korean hepatocellular carcinoma cell lines by cross-species color banding and comparative genomic hybridization
AU - Hwang, Hye Jin
AU - Kim, Gi Jin
AU - Lee, Gi Bong
AU - Oh, Jung Tak
AU - Chun, Yong Hyuck
AU - Park, Sun-Hwa
PY - 2003/3/1
Y1 - 2003/3/1
N2 - Chromosomal aberrations were investigated in hepatitis B virus integrated into the hepatocellular carcinoma (HCC) cell lines SNU-368, SNU-449, SNU-398, SNU-182, and SNU-475 using Giemsa-banding, cross species color banding, and comparative genomic hybridization (CGH). The origins of the marker chromosomes were confirmed by fluorescence in situ hybridization with constructed chromosome painting probes. Each cell line had unique modal karyotypic characteristics and showed variable numbers of numerical and structural clonal cytogenetic aberrations. The gains were commonly detected on chromosome 1, and chromosome regions 6p, 7q, 8q, 10p, 17q, and 20; the losses were often found on 4q21∼qter, 13, 18q21∼qter, and Y. In particular, the breakpoints on 1p36, 1p13∼q21, 2p13∼q11, 6q10∼q11, 7q11, 7q22, 14q10, 16q10∼q13, 17q21, 18q21, and 19p11∼q11 were involved frequently at the multiple rearranged lesions. CGH analysis further confirmed the cytogenetic data, and the nonrandom rearrangements data suggested the candidate regions for the genes to be isolated which were related to HCC.
AB - Chromosomal aberrations were investigated in hepatitis B virus integrated into the hepatocellular carcinoma (HCC) cell lines SNU-368, SNU-449, SNU-398, SNU-182, and SNU-475 using Giemsa-banding, cross species color banding, and comparative genomic hybridization (CGH). The origins of the marker chromosomes were confirmed by fluorescence in situ hybridization with constructed chromosome painting probes. Each cell line had unique modal karyotypic characteristics and showed variable numbers of numerical and structural clonal cytogenetic aberrations. The gains were commonly detected on chromosome 1, and chromosome regions 6p, 7q, 8q, 10p, 17q, and 20; the losses were often found on 4q21∼qter, 13, 18q21∼qter, and Y. In particular, the breakpoints on 1p36, 1p13∼q21, 2p13∼q11, 6q10∼q11, 7q11, 7q22, 14q10, 16q10∼q13, 17q21, 18q21, and 19p11∼q11 were involved frequently at the multiple rearranged lesions. CGH analysis further confirmed the cytogenetic data, and the nonrandom rearrangements data suggested the candidate regions for the genes to be isolated which were related to HCC.
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UR - http://www.scopus.com/inward/citedby.url?scp=0037372163&partnerID=8YFLogxK
U2 - 10.1016/S0165-4608(02)00671-4
DO - 10.1016/S0165-4608(02)00671-4
M3 - Article
VL - 141
SP - 128
EP - 137
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
SN - 0165-4608
IS - 2
ER -