A cystathionine-β-synthase domain-containing protein, CBSX2, regulates endothecial secondary cell wall thickening in anther development

Kwang Wook Jung, Yun Young Kim, Kyoung Shin Yoo, Sung Han Ok, Mei Hua Cui, Byung Cheon Jeong, Sang-Dong Yoo, Ji Ung Jeung, Jeong Sheop Shin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Anther formation and dehiscence are complex pivotal processes in reproductive development. The secondary wall thickening in endothecial cells of the anther is a known prerequisite for successful anther dehiscence. However, many gaps remain in our understanding of the regulatory mechanisms underlying anther dehiscence in planta, including a possible role for jasmonic acid (JA) and H2O2 in secondary wall thickening of endothecial cells. Here, we report that the cystathionine β-synthase domain-containing protein CBSX2 located in the chloroplast plays a critical role in thickening of the secondary cell walls of the endothecium during anther dehiscence in Arabidopsis. A T-DNA insertion mutant of CBSX2 (cbsx2) showed increased secondary wall thickening of endothecial cells and early anther dehiscence. Consistently, overexpression of CBSX2 resulted in anther indehiscence. Exogenous JA application induced secondary wall thickening and caused flower infertility in the cbsx2 mutant, whereas it partially restored fertility in the CBSX2-overexpressing lines lacking the wall thickening. CBSX2 directly modulated thioredoxin (Trx) in chloroplasts, which affected the level of H 2O2 and, consequently, expression of the genes involved in secondary cell wall thickening. Our findings have revealed that CBSX2 modulates the H2O2 status, which is linked to the JA response and in turn controls secondary wall thickening of the endothecial cells in anthers for dehiscence to occur.

Original languageEnglish
Pages (from-to)195-208
Number of pages14
JournalPlant and Cell Physiology
Volume54
Issue number2
DOIs
Publication statusPublished - 2013 Feb 1

Fingerprint

Cystathionine
cystathionine
anthers
Cell Wall
dehiscence
cell walls
Chloroplast Thioredoxins
jasmonic acid
proteins
Chloroplasts
Arabidopsis
Infertility
Fertility
chloroplasts
cells
Gene Expression
mutants
Protein Domains
Plantae
flowers

Keywords

  • Anther development
  • CBS domain-containing protein
  • HO2
  • Indehiscence
  • Jasmonic acid
  • Thioredoxin

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

A cystathionine-β-synthase domain-containing protein, CBSX2, regulates endothecial secondary cell wall thickening in anther development. / Jung, Kwang Wook; Kim, Yun Young; Yoo, Kyoung Shin; Ok, Sung Han; Cui, Mei Hua; Jeong, Byung Cheon; Yoo, Sang-Dong; Jeung, Ji Ung; Shin, Jeong Sheop.

In: Plant and Cell Physiology, Vol. 54, No. 2, 01.02.2013, p. 195-208.

Research output: Contribution to journalArticle

Jung, Kwang Wook ; Kim, Yun Young ; Yoo, Kyoung Shin ; Ok, Sung Han ; Cui, Mei Hua ; Jeong, Byung Cheon ; Yoo, Sang-Dong ; Jeung, Ji Ung ; Shin, Jeong Sheop. / A cystathionine-β-synthase domain-containing protein, CBSX2, regulates endothecial secondary cell wall thickening in anther development. In: Plant and Cell Physiology. 2013 ; Vol. 54, No. 2. pp. 195-208.
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