A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis

Zhizhong Gong, Chun Hai Dong, Hojoung Lee, Jianhua Zhu, Liming Xiong, Deming Gong, Becky Stevenson, Jian Kang Zhu

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

An Arabidopsis thaliana mutant, cryophyte, was isolated and found to have an enhanced cold stress-induction of the master regulator of cold tolerance, C-repeat binding factor 2 (CBF2), and its downstream target genes. The mutant is more tolerant to chilling and freezing stresses but is more sensitive to heat stress. Under warm but not cold growth temperatures, the mutant has a reduced stature and flowers earlier. Under long day conditions, flowering of the mutant is insensitive to vernalization. The mutant is also hypersensitive to the phytohormone abscisic acid. The mutation was found in a DEAD box RNA helicase gene that is identical to the previously identified low expression of osmotically responsive genes 4 (LOS4) locus, which was defined by the los4-1 mutation that reduces cold regulation of CBFs and their target genes and renders Arabidopsis plants chilling sensitive. We show evidence suggesting that the CRYOPHYTE/LOS4 protein may be enriched in the nuclear rim. In situ poly(A) hybridization indicates that the export of poly(A)+ RNAs is blocked in the cryophyte/los4-2 mutant at warm or high temperatures but not at low temperatures, whereas the los4-1 mutation weakens mRNA export at both low and warm temperatures. These results demonstrate an important role of the CRYOPHYTE/LOS4 RNA helicase in mRNA export, plant development, and stress responses.

Original languageEnglish
Pages (from-to)256-267
Number of pages12
JournalPlant Cell
Volume17
Issue number1
DOIs
Publication statusPublished - 2005 Dec 1
Externally publishedYes

Fingerprint

DEAD-box RNA Helicases
Arabidopsis
stress response
Genes
Chilling
mutants
Messenger RNA
Mutation
Temperature
RNA Helicases
mutation
Abscisic Acid
Poly A
Plant Growth Regulators
Plant Development
temperature
Growth temperature
genes
Freezing
RNA helicases

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis. / Gong, Zhizhong; Dong, Chun Hai; Lee, Hojoung; Zhu, Jianhua; Xiong, Liming; Gong, Deming; Stevenson, Becky; Zhu, Jian Kang.

In: Plant Cell, Vol. 17, No. 1, 01.12.2005, p. 256-267.

Research output: Contribution to journalArticle

Gong, Zhizhong ; Dong, Chun Hai ; Lee, Hojoung ; Zhu, Jianhua ; Xiong, Liming ; Gong, Deming ; Stevenson, Becky ; Zhu, Jian Kang. / A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis. In: Plant Cell. 2005 ; Vol. 17, No. 1. pp. 256-267.
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