A description of mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids

Byoung Jun Kim, Bo Ram Kim, Joseph Jeong, Ji Hun Lim, Sang Hyuk Park, Seung Heon Lee, Chang Ki Kim, Yoon Hoh Kook, Bum Joon Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Three rapidly growing mycobacterial strains, MOTTH4W, MOTT36W T and MOTT68W, were isolated from the sputa of three independent Korean patients co-infected with Mycobacterium yongonense Type II strains. The 16S rRNA gene sequences of all three strains were unique, which were closest to that of Mycobacterium chelonae subsp. bovis KCTC 39630 T (99.9% similarity). Multilocus sequence typing analysis targeting 10 housekeeping genes including hsp65 and rpoB revealed the distinct phylogenetic location of these strains, which were clustered with M. chelonae subsp. chelonae ATCC 35752 T and M. chelonae subsp. bovis KCTC 39630 T . Phylogenetic analysis based on whole genome sequences revealed a 95.89% average nucleotide identity (ANI) value with M. chelonae subsp. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, phenotypic characteristics such as a smooth colony morphology and growth inhibition at 37 °C, distinct MALDI-TOF MS profiles of extracted total lipids due to surface glycopeptidolipids, and distinct drug susceptibility profiles further supported the taxonomic characterization of these strains as representing a novel subspecies of Mycobacterium chelonae. Mycobacterium chelonae subsp. gwanakae subsp. nov. is proposed and the type strain is MOTT36W T (=KCTC 29127 T =JCM 32454 T ).

Original languageEnglish
Article number003056
Pages (from-to)3772-3780
Number of pages9
JournalInternational journal of systematic and evolutionary microbiology
Volume68
Issue number12
DOIs
Publication statusPublished - 2018 Dec 1
Externally publishedYes

Fingerprint

Mycobacterium chelonae
new subspecies
Mycobacterium
phenotype
phylogenetics
Phenotype
gene
subspecies
targeting
Multilocus Sequence Typing
drug
genome
lipid
Nucleotides
nucleotides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Essential Genes
phylogeny
Sputum
rRNA Genes

Keywords

  • Glycopeptidolipid
  • Mycobacterium chelonae subsp. gwanakae
  • Mycobacterium chelonae-abscessus complex
  • Rapidly growing mycobacteria
  • Smooth colony phenotype

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

A description of mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids. / Kim, Byoung Jun; Kim, Bo Ram; Jeong, Joseph; Lim, Ji Hun; Park, Sang Hyuk; Lee, Seung Heon; Kim, Chang Ki; Kook, Yoon Hoh; Kim, Bum Joon.

In: International journal of systematic and evolutionary microbiology, Vol. 68, No. 12, 003056, 01.12.2018, p. 3772-3780.

Research output: Contribution to journalArticle

Kim, Byoung Jun ; Kim, Bo Ram ; Jeong, Joseph ; Lim, Ji Hun ; Park, Sang Hyuk ; Lee, Seung Heon ; Kim, Chang Ki ; Kook, Yoon Hoh ; Kim, Bum Joon. / A description of mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids. In: International journal of systematic and evolutionary microbiology. 2018 ; Vol. 68, No. 12. pp. 3772-3780.
@article{a89813548bf44e37bf888e6ff8937aae,
title = "A description of mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids",
abstract = "Three rapidly growing mycobacterial strains, MOTTH4W, MOTT36W T and MOTT68W, were isolated from the sputa of three independent Korean patients co-infected with Mycobacterium yongonense Type II strains. The 16S rRNA gene sequences of all three strains were unique, which were closest to that of Mycobacterium chelonae subsp. bovis KCTC 39630 T (99.9{\%} similarity). Multilocus sequence typing analysis targeting 10 housekeeping genes including hsp65 and rpoB revealed the distinct phylogenetic location of these strains, which were clustered with M. chelonae subsp. chelonae ATCC 35752 T and M. chelonae subsp. bovis KCTC 39630 T . Phylogenetic analysis based on whole genome sequences revealed a 95.89{\%} average nucleotide identity (ANI) value with M. chelonae subsp. chelonae, slightly higher than the 95.0{\%} ANI criterion for determining a novel species. In addition, phenotypic characteristics such as a smooth colony morphology and growth inhibition at 37 °C, distinct MALDI-TOF MS profiles of extracted total lipids due to surface glycopeptidolipids, and distinct drug susceptibility profiles further supported the taxonomic characterization of these strains as representing a novel subspecies of Mycobacterium chelonae. Mycobacterium chelonae subsp. gwanakae subsp. nov. is proposed and the type strain is MOTT36W T (=KCTC 29127 T =JCM 32454 T ).",
keywords = "Glycopeptidolipid, Mycobacterium chelonae subsp. gwanakae, Mycobacterium chelonae-abscessus complex, Rapidly growing mycobacteria, Smooth colony phenotype",
author = "Kim, {Byoung Jun} and Kim, {Bo Ram} and Joseph Jeong and Lim, {Ji Hun} and Park, {Sang Hyuk} and Lee, {Seung Heon} and Kim, {Chang Ki} and Kook, {Yoon Hoh} and Kim, {Bum Joon}",
year = "2018",
month = "12",
day = "1",
doi = "10.1099/ijsem.0.003056",
language = "English",
volume = "68",
pages = "3772--3780",
journal = "International Journal of Systematic Bacteriology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "12",

}

TY - JOUR

T1 - A description of mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids

AU - Kim, Byoung Jun

AU - Kim, Bo Ram

AU - Jeong, Joseph

AU - Lim, Ji Hun

AU - Park, Sang Hyuk

AU - Lee, Seung Heon

AU - Kim, Chang Ki

AU - Kook, Yoon Hoh

AU - Kim, Bum Joon

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Three rapidly growing mycobacterial strains, MOTTH4W, MOTT36W T and MOTT68W, were isolated from the sputa of three independent Korean patients co-infected with Mycobacterium yongonense Type II strains. The 16S rRNA gene sequences of all three strains were unique, which were closest to that of Mycobacterium chelonae subsp. bovis KCTC 39630 T (99.9% similarity). Multilocus sequence typing analysis targeting 10 housekeeping genes including hsp65 and rpoB revealed the distinct phylogenetic location of these strains, which were clustered with M. chelonae subsp. chelonae ATCC 35752 T and M. chelonae subsp. bovis KCTC 39630 T . Phylogenetic analysis based on whole genome sequences revealed a 95.89% average nucleotide identity (ANI) value with M. chelonae subsp. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, phenotypic characteristics such as a smooth colony morphology and growth inhibition at 37 °C, distinct MALDI-TOF MS profiles of extracted total lipids due to surface glycopeptidolipids, and distinct drug susceptibility profiles further supported the taxonomic characterization of these strains as representing a novel subspecies of Mycobacterium chelonae. Mycobacterium chelonae subsp. gwanakae subsp. nov. is proposed and the type strain is MOTT36W T (=KCTC 29127 T =JCM 32454 T ).

AB - Three rapidly growing mycobacterial strains, MOTTH4W, MOTT36W T and MOTT68W, were isolated from the sputa of three independent Korean patients co-infected with Mycobacterium yongonense Type II strains. The 16S rRNA gene sequences of all three strains were unique, which were closest to that of Mycobacterium chelonae subsp. bovis KCTC 39630 T (99.9% similarity). Multilocus sequence typing analysis targeting 10 housekeeping genes including hsp65 and rpoB revealed the distinct phylogenetic location of these strains, which were clustered with M. chelonae subsp. chelonae ATCC 35752 T and M. chelonae subsp. bovis KCTC 39630 T . Phylogenetic analysis based on whole genome sequences revealed a 95.89% average nucleotide identity (ANI) value with M. chelonae subsp. chelonae, slightly higher than the 95.0% ANI criterion for determining a novel species. In addition, phenotypic characteristics such as a smooth colony morphology and growth inhibition at 37 °C, distinct MALDI-TOF MS profiles of extracted total lipids due to surface glycopeptidolipids, and distinct drug susceptibility profiles further supported the taxonomic characterization of these strains as representing a novel subspecies of Mycobacterium chelonae. Mycobacterium chelonae subsp. gwanakae subsp. nov. is proposed and the type strain is MOTT36W T (=KCTC 29127 T =JCM 32454 T ).

KW - Glycopeptidolipid

KW - Mycobacterium chelonae subsp. gwanakae

KW - Mycobacterium chelonae-abscessus complex

KW - Rapidly growing mycobacteria

KW - Smooth colony phenotype

UR - http://www.scopus.com/inward/record.url?scp=85059241990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059241990&partnerID=8YFLogxK

U2 - 10.1099/ijsem.0.003056

DO - 10.1099/ijsem.0.003056

M3 - Article

C2 - 30311876

AN - SCOPUS:85059241990

VL - 68

SP - 3772

EP - 3780

JO - International Journal of Systematic Bacteriology

JF - International Journal of Systematic Bacteriology

SN - 1466-5026

IS - 12

M1 - 003056

ER -