A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids

Jin Hui Joo; Miae Won; Sun Young Park; Kyungeun Park; Dong Sik Shin; Jong Seung Kim; Min Hee Lee

doi:10.1016/j.snb.2020.128360

A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids

Jin Hui Joo, Miae Won, Sun Young Park, Kyungeun Park, Dong Sik Shin, Jong Seung Kim, Min Hee Lee

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We present here a dicyanocoumarin-fused quinolinium based fluorescent probe to visualize NAD(P)H in living cells and tumor spheroids. The probe displays an NAD(P)H-selective strong ICT emission in a range of 600 ∼ 750 nm. In addition, the probe can provide strong fluorescence in response to a glycolysis-induced NADH promotion in living cells. Moreover, the probe was utilized to indicate cisplatin-induced glycolysis inhibition in breast cancer cells. Furthermore, the probe can detect hypoxic cancer cells and it was successfully applied to detect hypoxia in the inner region of breast tumor tissue mimicking 3D spheroids.

Original language	English
Article number	128360
Journal	Sensors and Actuators, B: Chemical
Volume	320
DOIs	https://doi.org/10.1016/j.snb.2020.128360
Publication status	Published - 2020 Oct 1

Keywords

Breast tumor spheroids
Dicyanocoumarin-fused quinolinium
Far-red light-emitting chemical probe
Glycolysis
Hypoxia
NAD(P)H detection

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2020.128360

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title = "A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids",

abstract = "We present here a dicyanocoumarin-fused quinolinium based fluorescent probe to visualize NAD(P)H in living cells and tumor spheroids. The probe displays an NAD(P)H-selective strong ICT emission in a range of 600 ∼ 750 nm. In addition, the probe can provide strong fluorescence in response to a glycolysis-induced NADH promotion in living cells. Moreover, the probe was utilized to indicate cisplatin-induced glycolysis inhibition in breast cancer cells. Furthermore, the probe can detect hypoxic cancer cells and it was successfully applied to detect hypoxia in the inner region of breast tumor tissue mimicking 3D spheroids.",

keywords = "Breast tumor spheroids, Dicyanocoumarin-fused quinolinium, Far-red light-emitting chemical probe, Glycolysis, Hypoxia, NAD(P)H detection",

author = "Joo, {Jin Hui} and Miae Won and Park, {Sun Young} and Kyungeun Park and Shin, {Dong Sik} and Kim, {Jong Seung} and Lee, {Min Hee}",

note = "Funding Information: This work was supported by CRI project no. 2018R1A3B1052702 (J. S. K.), no. 2019R1F1A1062208 (D.-S. S.) and no. 2018R1C1B6006110 (M. H. L.), of National Research Foundation (NRF) grant funded by Korea government. Funding Information: This work was supported by CRI project no. 2018R1A3B1052702 (J. S. K.), no. 2019R1F1A1062208 (D.-S. S.) and no. 2018R1C1B6006110 (M. H. L.), of National Research Foundation (NRF) grant funded by Korea government . Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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doi = "10.1016/j.snb.2020.128360",

language = "English",

volume = "320",

journal = "Sensors and Actuators, B: Chemical",

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T1 - A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids

AU - Joo, Jin Hui

AU - Won, Miae

AU - Park, Sun Young

AU - Park, Kyungeun

AU - Shin, Dong Sik

AU - Kim, Jong Seung

AU - Lee, Min Hee

N1 - Funding Information: This work was supported by CRI project no. 2018R1A3B1052702 (J. S. K.), no. 2019R1F1A1062208 (D.-S. S.) and no. 2018R1C1B6006110 (M. H. L.), of National Research Foundation (NRF) grant funded by Korea government. Funding Information: This work was supported by CRI project no. 2018R1A3B1052702 (J. S. K.), no. 2019R1F1A1062208 (D.-S. S.) and no. 2018R1C1B6006110 (M. H. L.), of National Research Foundation (NRF) grant funded by Korea government . Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/10/1

Y1 - 2020/10/1

N2 - We present here a dicyanocoumarin-fused quinolinium based fluorescent probe to visualize NAD(P)H in living cells and tumor spheroids. The probe displays an NAD(P)H-selective strong ICT emission in a range of 600 ∼ 750 nm. In addition, the probe can provide strong fluorescence in response to a glycolysis-induced NADH promotion in living cells. Moreover, the probe was utilized to indicate cisplatin-induced glycolysis inhibition in breast cancer cells. Furthermore, the probe can detect hypoxic cancer cells and it was successfully applied to detect hypoxia in the inner region of breast tumor tissue mimicking 3D spheroids.

AB - We present here a dicyanocoumarin-fused quinolinium based fluorescent probe to visualize NAD(P)H in living cells and tumor spheroids. The probe displays an NAD(P)H-selective strong ICT emission in a range of 600 ∼ 750 nm. In addition, the probe can provide strong fluorescence in response to a glycolysis-induced NADH promotion in living cells. Moreover, the probe was utilized to indicate cisplatin-induced glycolysis inhibition in breast cancer cells. Furthermore, the probe can detect hypoxic cancer cells and it was successfully applied to detect hypoxia in the inner region of breast tumor tissue mimicking 3D spheroids.

KW - Breast tumor spheroids

KW - Dicyanocoumarin-fused quinolinium

KW - Far-red light-emitting chemical probe

KW - Glycolysis

KW - Hypoxia

KW - NAD(P)H detection

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SN - 0925-4005

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JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

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A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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