A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids

Jin Hui Joo, Miae Won, Sun Young Park, Kyungeun Park, Dong Sik Shin, Jong Seung Kim, Min Hee Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We present here a dicyanocoumarin-fused quinolinium based fluorescent probe to visualize NAD(P)H in living cells and tumor spheroids. The probe displays an NAD(P)H-selective strong ICT emission in a range of 600 ∼ 750 nm. In addition, the probe can provide strong fluorescence in response to a glycolysis-induced NADH promotion in living cells. Moreover, the probe was utilized to indicate cisplatin-induced glycolysis inhibition in breast cancer cells. Furthermore, the probe can detect hypoxic cancer cells and it was successfully applied to detect hypoxia in the inner region of breast tumor tissue mimicking 3D spheroids.

Original languageEnglish
Article number128360
JournalSensors and Actuators, B: Chemical
Volume320
DOIs
Publication statusPublished - 2020 Oct 1

Keywords

  • Breast tumor spheroids
  • Dicyanocoumarin-fused quinolinium
  • Far-red light-emitting chemical probe
  • Glycolysis
  • Hypoxia
  • NAD(P)H detection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Fingerprint Dive into the research topics of 'A dicyanocoumarin-fused quinolinium based probe for NAD(P)H and its use for detecting glycolysis and hypoxia in living cells and tumor spheroids'. Together they form a unique fingerprint.

  • Cite this