A dielectric biosensor using the capacitance change with AC frequency integrated on glass substrates

Jongin Hong, Dae Sung Yoon, Myung Il Park, Jongwan Choi, Tae Song Kim, Geunbae Im, Sanghyo Kim, Y. Eugene Pak, Kwangsoo No

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Glass-based microchannel chips were fabricated using photolithographic technology, and Pt thin-film microelectrodes as dielectric biosensors were integrated on them. From capacitance-frequency measurements at various interelectrode distances and ionic concentrations, a significant difference between deionized (DI) water and tris-ethylenediaminetetraacetic acid (EDTA) (TE) buffer was observed in the low-frequency region. Although the capacitance (CM) of the DI water decreased as the interelectrode distance increased, that of the TE buffer was similar up to a frequency of 100 Hz, after which it was spilt in the same manner as the DI water above 100 Hz. As the ionic concentration increased, the CM of the TE buffer increased and the slope in the low frequency region changed from -0.875 to -0.425. The point where the slope changed shifted towards the frequency increase. These observations were clarified from the viewpoint of interfacial phenomena, such as the electrical double layer and Faradaic reactions, the dielectric constant related to conductivity, and the capacitance inversely proportional to the interelectrode distance. The addition of deoxyribonucleic acid (DNA) molecules (10ng/ul) increased the capacitance and dielectric loss in the TE buffer at low frequency. It is feasible to use dielectric properties for the rapid and direct detection of biomolecules, particularly DNA molecules, without using labels or indicators.

Original languageEnglish
Pages (from-to)5639-5645
Number of pages7
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume43
Issue number8 A
DOIs
Publication statusPublished - 2004 Aug 1
Externally publishedYes

Fingerprint

bioinstrumentation
Biosensors
Deionized water
alternating current
Capacitance
buffers
capacitance
Glass
glass
Substrates
low frequencies
DNA
deoxyribonucleic acid
water
slopes
Molecules
ethylenediaminetetraacetic acids
Microelectrodes
Ethylenediaminetetraacetic acid
frequency measurement

Keywords

  • Capacitance
  • Dielectric biosensor
  • Dielectric loss
  • DNA detection
  • Electrical double layer

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

A dielectric biosensor using the capacitance change with AC frequency integrated on glass substrates. / Hong, Jongin; Yoon, Dae Sung; Park, Myung Il; Choi, Jongwan; Kim, Tae Song; Im, Geunbae; Kim, Sanghyo; Eugene Pak, Y.; No, Kwangsoo.

In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers, Vol. 43, No. 8 A, 01.08.2004, p. 5639-5645.

Research output: Contribution to journalArticle

Hong, Jongin ; Yoon, Dae Sung ; Park, Myung Il ; Choi, Jongwan ; Kim, Tae Song ; Im, Geunbae ; Kim, Sanghyo ; Eugene Pak, Y. ; No, Kwangsoo. / A dielectric biosensor using the capacitance change with AC frequency integrated on glass substrates. In: Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers. 2004 ; Vol. 43, No. 8 A. pp. 5639-5645.
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