A direct, multiplex biosensor platform for pathogen detection based on cross-linked polydiacetylene (PDA) supramolecules

Cheol Hee Park, Jun Pyo Kim, Sang Wook Lee, Noo Lee Jeon, Pil J. Yoo, Sang Jun Sim

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

This study focuses on the development of a multiplex pathogen-detection platform based on poiydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 my, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.

Original languageEnglish
Pages (from-to)3703-3710
Number of pages8
JournalAdvanced Functional Materials
Volume19
Issue number23
DOIs
Publication statusPublished - 2009 Sep 12
Externally publishedYes

Fingerprint

pathogens
Liposomes
Pathogens
bioinstrumentation
Biosensors
platforms
ethylenediamine
fluorescence
immobilization
food
Fluorescence
chips
sensors
water
polydiacetylene
Sensors
Substrates
Water

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

A direct, multiplex biosensor platform for pathogen detection based on cross-linked polydiacetylene (PDA) supramolecules. / Park, Cheol Hee; Kim, Jun Pyo; Lee, Sang Wook; Jeon, Noo Lee; Yoo, Pil J.; Sim, Sang Jun.

In: Advanced Functional Materials, Vol. 19, No. 23, 12.09.2009, p. 3703-3710.

Research output: Contribution to journalArticle

Park, Cheol Hee ; Kim, Jun Pyo ; Lee, Sang Wook ; Jeon, Noo Lee ; Yoo, Pil J. ; Sim, Sang Jun. / A direct, multiplex biosensor platform for pathogen detection based on cross-linked polydiacetylene (PDA) supramolecules. In: Advanced Functional Materials. 2009 ; Vol. 19, No. 23. pp. 3703-3710.
@article{df98712c01b341e792e9b56619fc3063,
title = "A direct, multiplex biosensor platform for pathogen detection based on cross-linked polydiacetylene (PDA) supramolecules",
abstract = "This study focuses on the development of a multiplex pathogen-detection platform based on poiydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 my, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.",
author = "Park, {Cheol Hee} and Kim, {Jun Pyo} and Lee, {Sang Wook} and Jeon, {Noo Lee} and Yoo, {Pil J.} and Sim, {Sang Jun}",
year = "2009",
month = "9",
day = "12",
doi = "10.1002/adfm.200900393",
language = "English",
volume = "19",
pages = "3703--3710",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley-VCH Verlag",
number = "23",

}

TY - JOUR

T1 - A direct, multiplex biosensor platform for pathogen detection based on cross-linked polydiacetylene (PDA) supramolecules

AU - Park, Cheol Hee

AU - Kim, Jun Pyo

AU - Lee, Sang Wook

AU - Jeon, Noo Lee

AU - Yoo, Pil J.

AU - Sim, Sang Jun

PY - 2009/9/12

Y1 - 2009/9/12

N2 - This study focuses on the development of a multiplex pathogen-detection platform based on poiydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 my, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.

AB - This study focuses on the development of a multiplex pathogen-detection platform based on poiydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 my, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.

UR - http://www.scopus.com/inward/record.url?scp=72649087988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72649087988&partnerID=8YFLogxK

U2 - 10.1002/adfm.200900393

DO - 10.1002/adfm.200900393

M3 - Article

AN - SCOPUS:72649087988

VL - 19

SP - 3703

EP - 3710

JO - Advanced Functional Materials

JF - Advanced Functional Materials

SN - 1616-301X

IS - 23

ER -