A direct, multiplex biosensor platform for pathogen detection based on cross-linked polydiacetylene (PDA) supramolecules

Cheol Hee Park, Jun Pyo Kim, Sang Wook Lee, Noo Lee Jeon, Pil J. Yoo, Sang Jun Sim

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

This study focuses on the development of a multiplex pathogen-detection platform based on poiydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 my, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.

Original languageEnglish
Pages (from-to)3703-3710
Number of pages8
JournalAdvanced Functional Materials
Volume19
Issue number23
DOIs
Publication statusPublished - 2009 Sep 12

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrochemistry

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