A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system

Eun Hee Lee, Hyun Jeong Lim, Ahjeong Son, Beelee Chua

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min-1 and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS).

Original languageEnglish
Pages (from-to)7776-7783
Number of pages8
JournalAnalyst
Volume140
Issue number22
DOIs
Publication statusPublished - 2015 Nov 21

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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