A distinctive role for focal adhesion proteins in three-dimensional cell motility

Stephanie I. Fraley, Yunfeng Feng, Ranjini Krishnamurthy, Dong-Hwee Kim, Alfredo Celedon, Gregory D. Longmore, Denis Wirtz

Research output: Contribution to journalArticle

344 Citations (Scopus)

Abstract

Focal adhesions are large multi-protein assemblies that form at the basal surface of cells on planar dishes, and that mediate cell signalling, force transduction and adhesion to the substratum. Although much is known about focal adhesion components in two-dimensional (2D) systems, their role in migrating cells in a more physiological three-dimensional (3D) matrix is largely unknown. Live-cell microscopy shows that for cells fully embedded in a 3D matrix, focal adhesion proteins, including vinculin, paxillin, talin, α-actinin, zyxin, VASP, FAK and p130Cas, do not form aggregates but are diffusely distributed throughout the cytoplasm. Despite the absence of detectable focal adhesions, focal adhesion proteins still modulate cell motility, but in a manner distinct from cells on planar substrates. Rather, focal adhesion proteins in matrix-embedded cells regulate cell speed and persistence by affecting protrusion activity and matrix deformation, two processes that have no direct role in controlling 2D cell speed. This study shows that membrane protrusions constitute a critical motility/matrix-traction module that drives cell motility in a 3D matrix.

Original languageEnglish
Pages (from-to)598-604
Number of pages7
JournalNature Cell Biology
Volume12
Issue number6
DOIs
Publication statusPublished - 2010 Jun 1
Externally publishedYes

Fingerprint

Focal Adhesions
Cell Movement
Proteins
Zyxin
Talin
Paxillin
Vinculin
Actinin
Traction
Microscopy
Cytoplasm
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fraley, S. I., Feng, Y., Krishnamurthy, R., Kim, D-H., Celedon, A., Longmore, G. D., & Wirtz, D. (2010). A distinctive role for focal adhesion proteins in three-dimensional cell motility. Nature Cell Biology, 12(6), 598-604. https://doi.org/10.1038/ncb2062

A distinctive role for focal adhesion proteins in three-dimensional cell motility. / Fraley, Stephanie I.; Feng, Yunfeng; Krishnamurthy, Ranjini; Kim, Dong-Hwee; Celedon, Alfredo; Longmore, Gregory D.; Wirtz, Denis.

In: Nature Cell Biology, Vol. 12, No. 6, 01.06.2010, p. 598-604.

Research output: Contribution to journalArticle

Fraley, SI, Feng, Y, Krishnamurthy, R, Kim, D-H, Celedon, A, Longmore, GD & Wirtz, D 2010, 'A distinctive role for focal adhesion proteins in three-dimensional cell motility', Nature Cell Biology, vol. 12, no. 6, pp. 598-604. https://doi.org/10.1038/ncb2062
Fraley SI, Feng Y, Krishnamurthy R, Kim D-H, Celedon A, Longmore GD et al. A distinctive role for focal adhesion proteins in three-dimensional cell motility. Nature Cell Biology. 2010 Jun 1;12(6):598-604. https://doi.org/10.1038/ncb2062
Fraley, Stephanie I. ; Feng, Yunfeng ; Krishnamurthy, Ranjini ; Kim, Dong-Hwee ; Celedon, Alfredo ; Longmore, Gregory D. ; Wirtz, Denis. / A distinctive role for focal adhesion proteins in three-dimensional cell motility. In: Nature Cell Biology. 2010 ; Vol. 12, No. 6. pp. 598-604.
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