A divergent external loop confers antagonistic activity on floral regulators FT and TFL1

Ji Hoon Ahn, David Miller, Victoria J. Winter, Mark J. Banfield, Hwan Lee Jeong, Yeon Yoo So, Stefan R. Henz, Robert Leo Brady, Detlef Weigel

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

The Arabidopsis genes FT and TERMINAL FLOWER1 (TFL1) encode related proteins with similarity to human Raf kinase inhibitor protein. FT, and likely also TFL1, is recruited to the promoters of floral genes through interaction with FD, a bZIP transcription factor. FT, however, induces flowering, while TFL1 represses flowering. Residues responsible for the opposite activities of FT and TFL1 were mapped by examining plants that overexpress chimeric proteins. A region important in vivo localizes to a 14-amino-acid segment that evolves very rapidly in TFL1 orthologs, but is almost invariant in FT orthologs. Crystal structures show that this segment forms an external loop of variable conformation. The only residue unambiguously distinguishing the FT and TFL1 loops makes a hydrogen bond with a residue near the entrance of a potential ligand-binding pocket in TFL1, but not in FT. This pocket is contacted by a C-terminal peptide, which also contributes to the opposite FT and TFL1 activities. In combination, these results identify a molecular surface likely to be recognized by FT- and/or TFL1-specific interactors.

Original languageEnglish
Pages (from-to)605-614
Number of pages10
JournalEMBO Journal
Volume25
Issue number3
DOIs
Publication statusPublished - 2006 Feb 8

Keywords

  • Florigen
  • Flowering
  • PEBP
  • RKIP
  • Raf kinase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Ahn, J. H., Miller, D., Winter, V. J., Banfield, M. J., Jeong, H. L., So, Y. Y., Henz, S. R., Brady, R. L., & Weigel, D. (2006). A divergent external loop confers antagonistic activity on floral regulators FT and TFL1. EMBO Journal, 25(3), 605-614. https://doi.org/10.1038/sj.emboj.7600950