A DNA - Gold nanoparticle-based colorimetric competition assay for the detection of cysteine

Jae-Seung Lee, Pirmin A. Ulmann, Min Su Han, Chad A. Mirkin

Research output: Contribution to journalArticle

408 Citations (Scopus)

Abstract

We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg 2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg 2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.

Original languageEnglish
Pages (from-to)529-533
Number of pages5
JournalNano Letters
Volume8
Issue number2
DOIs
Publication statusPublished - 2008 Feb 1
Externally publishedYes

Fingerprint

cysteine
Gold
Cysteine
thymidine
Assays
DNA
deoxyribonucleic acid
oligonucleotides
Oligonucleotides
gold
Nanoparticles
nanoparticles
Thymidine
Ultraviolet spectrometers
Melting
Coloring Agents
Dyes
Optical properties
dyes
melting

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

A DNA - Gold nanoparticle-based colorimetric competition assay for the detection of cysteine. / Lee, Jae-Seung; Ulmann, Pirmin A.; Han, Min Su; Mirkin, Chad A.

In: Nano Letters, Vol. 8, No. 2, 01.02.2008, p. 529-533.

Research output: Contribution to journalArticle

Lee, Jae-Seung ; Ulmann, Pirmin A. ; Han, Min Su ; Mirkin, Chad A. / A DNA - Gold nanoparticle-based colorimetric competition assay for the detection of cysteine. In: Nano Letters. 2008 ; Vol. 8, No. 2. pp. 529-533.
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