A dominant antigenic region of the Hantaan virus nucleocapsid protein is located within a amino-terminal short stretch of hydrophilic residues

J. I. Kang, Y. S. Lee, K. Ahn, J. L. Song, K. J. Song, L. J. Baek, P. W. Lee, B. Y. Ahn

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The nucleocapsid (N) protein of the Hantaan virus (HTNV) is a major viral antigen that induces a strong antibody response during the acute phase of infection. By immunoblot analyses of the recombinant N proteins using human sera of the hemorrhagic fever with renal syndrome (HFRS), we have confirmed previous finding by other investigators of the presence of a highly antigenic region near the amino terminus of the HTNV N protein. We have further located the antigenic region within a short stretch of hydrophilic sequences between the 26 and the 46th amino acid residues. The recombinant glutathione S-transferase fusion proteins containing this region was expressed as a soluble form in a large quantity in Escherichia coli, and purified by a single-step affinity chromatography. The recombinant antigen also showed a similar, but a weaker reactivity with human antisera to Seoul virus (SEOV), the virus most closely related to HTNV.

Original languageEnglish
Pages (from-to)183-186
Number of pages4
JournalVirus Genes
Volume23
Issue number2
DOIs
Publication statusPublished - 2001

Keywords

  • Antigenicity
  • HFRS
  • Hantaan virus
  • Nucleocapsid
  • Recombinant protein
  • Seoul virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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