A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture

Seung Kwan Moon, Myeong Jin Oh, Dong Hyun Paik, Tae Kyung Ryu, Kyeongsoon Park, Sung Eun Kim, Jong Hoon Park, Jung Hyun Kim, Sung Wook Choi

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.

    Original languageEnglish
    Pages (from-to)399-405
    Number of pages7
    JournalMacromolecular Rapid Communications
    Volume34
    Issue number5
    DOIs
    Publication statusPublished - 2013 Mar 12

    Keywords

    • cell culture
    • fluidic devices
    • monodisperse beads
    • porous materials
    • uniform pore size

    ASJC Scopus subject areas

    • Organic Chemistry
    • Polymers and Plastics
    • Materials Chemistry

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