A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture

Seung Kwan Moon, Myeong Jin Oh, Dong Hyun Paik, Tae Kyung Ryu, Kyeongsoon Park, Sung Eun Kim, Jong Hoon Park, Jung Hyun Kim, Sung Wook Choi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

This paper describes a facile method for the preparation of porous gelatin beads with uniform pore sizes using a simple fluidic device and their application as supporting materials for cell culture. An aqueous gelatin droplet containing many uniform toluene droplets, produced in the fluidic device, is dropped into liquid nitrogen for instant freezing and the small toluene droplets evolve into pores in the gelatin beads after removal of toluene and then freeze-drying. The porous gelatin beads exhibit a uniform pore size and monodisperse diameter as well as large open pores at the surface. Fluorescence microscopy images of fibroblast-loaded gelatin beads confirm the attachment and proliferation of the cells throughout the porous gelatin beads.

Original languageEnglish
Pages (from-to)399-405
Number of pages7
JournalMacromolecular Rapid Communications
Volume34
Issue number5
DOIs
Publication statusPublished - 2013 Mar 11

Keywords

  • cell culture
  • fluidic devices
  • monodisperse beads
  • porous materials
  • uniform pore size

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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  • Cite this

    Moon, S. K., Oh, M. J., Paik, D. H., Ryu, T. K., Park, K., Kim, S. E., Park, J. H., Kim, J. H., & Choi, S. W. (2013). A facile method for the preparation of monodisperse beads with uniform pore sizes for cell culture. Macromolecular Rapid Communications, 34(5), 399-405. https://doi.org/10.1002/marc.201200711