A far-red, photo- and bio-stable fluorescent marker selective to the endoplasmic reticulum and its application to tunicamycin-treated HeLa cells

Hye Ri Kim; Rajesh Kumar; Woori Kim; Jae Hong Lee; Myungkoo Suh; Amit Sharma; Chang Hoe Kim; Chulhun Kang; Jong Seung Kim

doi:10.1039/c6cc02330d

A far-red, photo- and bio-stable fluorescent marker selective to the endoplasmic reticulum and its application to tunicamycin-treated HeLa cells

Hye Ri Kim, Rajesh Kumar, Woori Kim, Jae Hong Lee, Myungkoo Suh, Amit Sharma, Chang Hoe Kim, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Herein, we developed an ER selective fluorescent ERp that exhibited a sharp fluorescence emission in the far-red region and high photo- and bio-stability in biological milieu. Its emission is insensitive to pH change and localized in the ER of the cells. Furthermore, it successfully demonstrated that the ER membrane is rapidly reorganized in the perinuclear region by an ER stress inducer, tunicamycin.

Original language	English
Pages (from-to)	7134-7137
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	44
DOIs	https://doi.org/10.1039/c6cc02330d
Publication status	Published - 2016

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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title = "A far-red, photo- and bio-stable fluorescent marker selective to the endoplasmic reticulum and its application to tunicamycin-treated HeLa cells",

abstract = "Herein, we developed an ER selective fluorescent ERp that exhibited a sharp fluorescence emission in the far-red region and high photo- and bio-stability in biological milieu. Its emission is insensitive to pH change and localized in the ER of the cells. Furthermore, it successfully demonstrated that the ER membrane is rapidly reorganized in the perinuclear region by an ER stress inducer, tunicamycin.",

author = "Kim, {Hye Ri} and Rajesh Kumar and Woori Kim and Lee, {Jae Hong} and Myungkoo Suh and Amit Sharma and Kim, {Chang Hoe} and Chulhun Kang and Kim, {Jong Seung}",

note = "Funding Information: This work was supported by CRI (No. 2009-0081566, JSK), NRF (No. 2014R1A2A1A11052325, CK) and the Basic Science Research Program (No. 20100020209) project grants from the National Research Foundation of Korea. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

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language = "English",

volume = "52",

pages = "7134--7137",

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T1 - A far-red, photo- and bio-stable fluorescent marker selective to the endoplasmic reticulum and its application to tunicamycin-treated HeLa cells

AU - Kim, Hye Ri

AU - Kumar, Rajesh

AU - Kim, Woori

AU - Lee, Jae Hong

AU - Suh, Myungkoo

AU - Sharma, Amit

AU - Kim, Chang Hoe

AU - Kang, Chulhun

AU - Kim, Jong Seung

N1 - Funding Information: This work was supported by CRI (No. 2009-0081566, JSK), NRF (No. 2014R1A2A1A11052325, CK) and the Basic Science Research Program (No. 20100020209) project grants from the National Research Foundation of Korea. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2016

Y1 - 2016

N2 - Herein, we developed an ER selective fluorescent ERp that exhibited a sharp fluorescence emission in the far-red region and high photo- and bio-stability in biological milieu. Its emission is insensitive to pH change and localized in the ER of the cells. Furthermore, it successfully demonstrated that the ER membrane is rapidly reorganized in the perinuclear region by an ER stress inducer, tunicamycin.

AB - Herein, we developed an ER selective fluorescent ERp that exhibited a sharp fluorescence emission in the far-red region and high photo- and bio-stability in biological milieu. Its emission is insensitive to pH change and localized in the ER of the cells. Furthermore, it successfully demonstrated that the ER membrane is rapidly reorganized in the perinuclear region by an ER stress inducer, tunicamycin.

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DO - 10.1039/c6cc02330d

M3 - Article

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AN - SCOPUS:84971320751

SN - 1359-7345

VL - 52

SP - 7134

EP - 7137

JO - Chemical Communications

JF - Chemical Communications

IS - 44

ER -

A far-red, photo- and bio-stable fluorescent marker selective to the endoplasmic reticulum and its application to tunicamycin-treated HeLa cells

Abstract

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