A Fluorescent Probe for Stimulated Emission Depletion Super-Resolution Imaging of Vicinal-Dithiol-Proteins on Mitochondrial Membrane

Zhigang Yang, Dong Hoon Kang, Hoyeon Lee, Jinwoo Shin, Wei Yan, Bhowmira Rathore, Hye Ri Kim, Seo Jin Kim, Hardev Singh, Liwei Liu, Junle Qu, Chulhun Kang, Jong Seung Kim

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Abstract

Realizing the significant roles of vicinal-dithiol proteins (VDPs) in maintaining the cellular redox homeostasis and their implication in many diseases, we synthesized a smart arsenate based fluorescent probe 1 which can preferentially target the mitochondrial membrane-bound vicinal dithiol proteins (VDPs), especially voltage-dependent anion channel (VDAC2). The probe targetability was demonstrated by in vitro studies such as colocalization, stimulated emission depletion (STED) super-resolution imaging, proteomic MS/MS analysis, and Western blot analysis. The probe represents a rare example of fluorescence labeling of mitochondrial membrane-bound VDPs and can provide a new way to construct VDPs-specific fluorescent probes to gain deeper understanding of their roles in mitochondrial-related disorders.

Original languageEnglish
Pages (from-to)1446-1453
Number of pages8
JournalBioconjugate Chemistry
Volume29
Issue number4
DOIs
Publication statusPublished - 2018 Apr 18

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Yang, Z., Kang, D. H., Lee, H., Shin, J., Yan, W., Rathore, B., Kim, H. R., Kim, S. J., Singh, H., Liu, L., Qu, J., Kang, C., & Kim, J. S. (2018). A Fluorescent Probe for Stimulated Emission Depletion Super-Resolution Imaging of Vicinal-Dithiol-Proteins on Mitochondrial Membrane. Bioconjugate Chemistry, 29(4), 1446-1453. https://doi.org/10.1021/acs.bioconjchem.8b00128