A fluorescent probe for the Fe3+ ion pool in endoplasmic reticulum in liver cells

Min Hee Lee, Hoyeon Lee, Min Jung Chang, Hyeong Seok Kim, Chulhun Kang, Jong Seung Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have presented rhodamine-derived Schiff base probes (1-6) selective of the Fe3+ ion in biological systems. In the presence of Fe3+ ions, the probes display new absorption and emission bands at 526 nm and 551 nm, respectively. Confocal microscopic analysis using the HepG2 cell line showed that probes 1-6 provide fluorescence Off-On changes caused by cellular Fe3+ ions, and demonstrated a largely enhanced fluorescence upon iron overloading of the cells. We also found that the Fe3+ ion-mediated fluorescence changes of probes 1-6 are mainly localized in the ER (Endoplasmic Reticulum) in HepG2 cells. Among them, probe 5 exhibits the highest selectivity for ER localization over the other organelles.

Original languageEnglish
Pages (from-to)245-250
Number of pages6
JournalDyes and Pigments
Volume130
DOIs
Publication statusPublished - 2016 Jul 1

Keywords

  • Cell imaging
  • Fluorescent probe
  • Iron detection
  • Rhodamine

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

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