A fluorescent probe to detect thiol-containing amino acids in solidtumors

Wen Xiu Ren, Jiyou Han, Tuhin Pradhan, Ja Yun Lim, Jae Hong Lee, Jaehun Lee, Jong-Hoon Kim, Jong Seung Kim

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Early detecting of cancer is critical to provide proper treatment and to improve survival of patients. Here, we reported a highly sensitive ratiometric (yellow emission (550nm) to blue emission (496nm)) fluorescent probe 1 developed for detection of thiol-containing amino acids. This probe successfully eliminates interference from background autofluorescence, and discriminates between human carcinoma and normal cells by detecting intracellular thiol levels in living cells (. P<0.05). Furthermore, the ability of the probe to identify growing tumors by measuring GSH in the tissues as well as in the fresh blood of tumor xenograft mice. Additionally, the ratio of the emission intensity at two different wavelengths can provide quantitative analysis of glutathione (GSH) in the living systems. It suggests that it represents a promising prognostic and diagnostic marker, with extensive and simple potential clinical applications.

Original languageEnglish
Pages (from-to)4157-4167
Number of pages11
JournalBiomaterials
Volume35
Issue number13
DOIs
Publication statusPublished - 2014 Apr 1

Fingerprint

Fluorescent Dyes
Sulfhydryl Compounds
Amino acids
Tumors
Amino Acids
Heterografts
Glutathione
Neoplasms
Blood
Cells
Tissue
Wavelength
Chemical analysis
Carcinoma
Survival
Therapeutics

Keywords

  • Blood
  • GSH measurement
  • Hepatocellular carcinoma
  • Ratiometric fluorescence
  • Tumor tissue

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

A fluorescent probe to detect thiol-containing amino acids in solidtumors. / Ren, Wen Xiu; Han, Jiyou; Pradhan, Tuhin; Lim, Ja Yun; Lee, Jae Hong; Lee, Jaehun; Kim, Jong-Hoon; Kim, Jong Seung.

In: Biomaterials, Vol. 35, No. 13, 01.04.2014, p. 4157-4167.

Research output: Contribution to journalArticle

Ren, Wen Xiu ; Han, Jiyou ; Pradhan, Tuhin ; Lim, Ja Yun ; Lee, Jae Hong ; Lee, Jaehun ; Kim, Jong-Hoon ; Kim, Jong Seung. / A fluorescent probe to detect thiol-containing amino acids in solidtumors. In: Biomaterials. 2014 ; Vol. 35, No. 13. pp. 4157-4167.
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