A fully microfabricated carbon nanotube three-electrode system on glass substrate for miniaturized electrochemical biosensors

Joon Hyub Kim, Jun Yong Lee, Joon Hyung Jin, Chan Won Park, Cheol Jin Lee, Nam Ki Min

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We present an integration process to fabricate single-walled carbon nanotube (SWCNT) three-electrode systems on glass substrate for electrochemical biosensors. Key issues involve optimization of the SWCNT working electrode to achieve high sensitivity, developing an optimal Ag/AgCl reference electrode with good stability, and process development to integrate these electrodes. Multiple spray coatings of the SWCNT film on glass substrate enabled easier integration of the SWCNT film into an electrochemical three-electrode system. O 2 plasma etching and subsequent activation of spray-coated SWCNT films were needed to pattern and functionalize the SWCNT working electrode films without serious damage to the SWCNTs, and to remove organic residues. The microfabricated threeelectrode systems were characterized by microscopic and spectroscopic techniques, and the electrochemical properties were investigated using cyclic voltammetry and chronoamperometry. The fully-integrated CNT three-electrode system showed an effective working electrode area about three times larger than its geometric surface area and an improved electrochemical activity for hydrogen peroxide decomposition. Finally, the effectiveness of miniaturized pf-SWCNT electrodes as biointerfaces was examined by applying them to immunosensors to detect Legionella(L) pneumophila, based on a direct sandwich enzyme-linked immunosorbent assay (ELISA) format with 3,3',5,5'-tetramethylbenzidine dihydrochloride/hydrogen peroxide(TMB/H 2O 2) as the substrate/ mediator system. The lower detection limit of the pf- SWCNT-based immunosensors to L. pneumophila is about 1500 times lower than that of the standard ELISA assay.

Original languageEnglish
Pages (from-to)613-624
Number of pages12
JournalBiomedical Microdevices
Volume14
Issue number3
DOIs
Publication statusPublished - 2012 Jun 1

Fingerprint

Carbon Nanotubes
Biosensing Techniques
Biosensors
Single-walled carbon nanotubes (SWCN)
Glass
Carbon nanotubes
Electrodes
Substrates
Immunosensors
Assays
Hydrogen peroxide
Hydrogen Peroxide
Enzymes
Enzyme-Linked Immunosorbent Assay
Electrochemical Techniques
Legionella pneumophila
Chronoamperometry
Plasma etching
Electrochemical properties
Cyclic voltammetry

Keywords

  • Biosensor
  • Plasma functionalization
  • Spray-coated SWCNT
  • Three electrode system

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

A fully microfabricated carbon nanotube three-electrode system on glass substrate for miniaturized electrochemical biosensors. / Kim, Joon Hyub; Lee, Jun Yong; Jin, Joon Hyung; Park, Chan Won; Lee, Cheol Jin; Min, Nam Ki.

In: Biomedical Microdevices, Vol. 14, No. 3, 01.06.2012, p. 613-624.

Research output: Contribution to journalArticle

Kim, Joon Hyub ; Lee, Jun Yong ; Jin, Joon Hyung ; Park, Chan Won ; Lee, Cheol Jin ; Min, Nam Ki. / A fully microfabricated carbon nanotube three-electrode system on glass substrate for miniaturized electrochemical biosensors. In: Biomedical Microdevices. 2012 ; Vol. 14, No. 3. pp. 613-624.
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