A gammaherpesvirus establishes persistent infection in neuroblastoma cells

Hye Jeong Cho, Moon Jung Song

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly understood. Here, we characterized a unique pattern of MHV-68 persistent infection in neuroblastoma cells. On infection with MHV-68, both murine and human neuroblastoma cells expressed viral lytic proteins and produced virions. However, the infected cells survived productive infection and could be cultured for multiple passages without affecting their cellular growth. Latent infection as well as productive replication was established in these prolonged cultures, and lytic replication was further increased by treatment with lytic inducers. Our results provide a novel system to study persistent infection of γHVs in vitro following de novo infection and suggest application of MHV-68 as a potential gene transfer vector to the brain.

Original languageEnglish
Pages (from-to)518-525
Number of pages8
JournalMolecules and Cells
Volume37
Issue number7
DOIs
Publication statusPublished - 2014 Jul 1

Fingerprint

Neuroblastoma
Infection
Brain
Central Nervous System Infections
Viral Proteins
Virion
Viruses
Growth
Genes

Keywords

  • CNS
  • gammaherpesvirus
  • gene delivery
  • neuroblastoma
  • persistent infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A gammaherpesvirus establishes persistent infection in neuroblastoma cells. / Cho, Hye Jeong; Song, Moon Jung.

In: Molecules and Cells, Vol. 37, No. 7, 01.07.2014, p. 518-525.

Research output: Contribution to journalArticle

@article{a62b48d2a96646c09b5d88c7dfef71c7,
title = "A gammaherpesvirus establishes persistent infection in neuroblastoma cells",
abstract = "Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly understood. Here, we characterized a unique pattern of MHV-68 persistent infection in neuroblastoma cells. On infection with MHV-68, both murine and human neuroblastoma cells expressed viral lytic proteins and produced virions. However, the infected cells survived productive infection and could be cultured for multiple passages without affecting their cellular growth. Latent infection as well as productive replication was established in these prolonged cultures, and lytic replication was further increased by treatment with lytic inducers. Our results provide a novel system to study persistent infection of γHVs in vitro following de novo infection and suggest application of MHV-68 as a potential gene transfer vector to the brain.",
keywords = "CNS, gammaherpesvirus, gene delivery, neuroblastoma, persistent infection",
author = "Cho, {Hye Jeong} and Song, {Moon Jung}",
year = "2014",
month = "7",
day = "1",
doi = "10.14348/molcells.2014.0024",
language = "English",
volume = "37",
pages = "518--525",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "7",

}

TY - JOUR

T1 - A gammaherpesvirus establishes persistent infection in neuroblastoma cells

AU - Cho, Hye Jeong

AU - Song, Moon Jung

PY - 2014/7/1

Y1 - 2014/7/1

N2 - Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly understood. Here, we characterized a unique pattern of MHV-68 persistent infection in neuroblastoma cells. On infection with MHV-68, both murine and human neuroblastoma cells expressed viral lytic proteins and produced virions. However, the infected cells survived productive infection and could be cultured for multiple passages without affecting their cellular growth. Latent infection as well as productive replication was established in these prolonged cultures, and lytic replication was further increased by treatment with lytic inducers. Our results provide a novel system to study persistent infection of γHVs in vitro following de novo infection and suggest application of MHV-68 as a potential gene transfer vector to the brain.

AB - Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly understood. Here, we characterized a unique pattern of MHV-68 persistent infection in neuroblastoma cells. On infection with MHV-68, both murine and human neuroblastoma cells expressed viral lytic proteins and produced virions. However, the infected cells survived productive infection and could be cultured for multiple passages without affecting their cellular growth. Latent infection as well as productive replication was established in these prolonged cultures, and lytic replication was further increased by treatment with lytic inducers. Our results provide a novel system to study persistent infection of γHVs in vitro following de novo infection and suggest application of MHV-68 as a potential gene transfer vector to the brain.

KW - CNS

KW - gammaherpesvirus

KW - gene delivery

KW - neuroblastoma

KW - persistent infection

UR - http://www.scopus.com/inward/record.url?scp=84929289940&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929289940&partnerID=8YFLogxK

U2 - 10.14348/molcells.2014.0024

DO - 10.14348/molcells.2014.0024

M3 - Article

C2 - 25092213

AN - SCOPUS:84929289940

VL - 37

SP - 518

EP - 525

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 7

ER -