A genome-wide analysis of transcripts in a 2BS.2RL wheat-rye translocation during Hessian fly infestation

Tong Geon Lee, Dae Yeon Kim, Jerry W. Johnson, Yong Weon Seo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Hessian fly [Mayetiola destructor (Say)] biotype L is the most damaging pest to wheat (Triticum aestivum L.). The long arm of rye chromosome 2 (2RL) has been transferred to common wheat, in the form of 2BS.2RL wheat-rye translocations. 2RL carries gene(s) for resistance to Hessian fly (biotype L), representing a promising target for engineered resistance. The objective of this study was to examine changes in gene expression in near-isogenic lines (NILs) differing by the presence (resistant NIL) or absence (susceptible NIL) of 2RL under Hessian fly infestation. Gene expression was detected using an Affymetrix 3′ IVT wheat genome array. Separate comparisons of data sets generated from the array were performed to identify transcripts involved in the defense pathways. Diverse resistant NIL-specific transcripts including ethylene-responsive factor-like transcription factors and zinc-finger proteins were discovered. Evidence of the enrichment of gene ontologies associated with hydrolase was found in resistant NIL during Hessian fly infestation. The gene information identified in this study would be an important resource in growing our understanding of the resistance mechanisms and for cloning candidate genes.

Original languageEnglish
Pages (from-to)795-803
Number of pages9
JournalGenes and Genomics
Volume35
Issue number6
DOIs
Publication statusPublished - 2013 Dec 1

Fingerprint

Diptera
Triticum
Genes
Genome
Gene expression
Gene Expression
Gene Ontology
Chromosomes, Human, Pair 2
Cloning
Zinc Fingers
Hydrolases
Chromosomes
Ontology
Zinc
Organism Cloning
Transcription Factors
Secale
Proteins

Keywords

  • Hessian fly
  • Resistance mechanisms
  • Wheat
  • Wheat genome array
  • Wheat-rye translocations

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Molecular Biology

Cite this

A genome-wide analysis of transcripts in a 2BS.2RL wheat-rye translocation during Hessian fly infestation. / Lee, Tong Geon; Kim, Dae Yeon; Johnson, Jerry W.; Seo, Yong Weon.

In: Genes and Genomics, Vol. 35, No. 6, 01.12.2013, p. 795-803.

Research output: Contribution to journalArticle

Lee, Tong Geon ; Kim, Dae Yeon ; Johnson, Jerry W. ; Seo, Yong Weon. / A genome-wide analysis of transcripts in a 2BS.2RL wheat-rye translocation during Hessian fly infestation. In: Genes and Genomics. 2013 ; Vol. 35, No. 6. pp. 795-803.
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