TY - JOUR
T1 - A genomic-scale view of the camp response element-enhancer decoy
T2 - A tumor target-based genetic tool
AU - Cho, Yee Sook
AU - Kim, Meyoung-Kon
AU - Cheadle, Chris
AU - Neary, Catherine
AU - Park, Yun Gyu
AU - Becker, Kevin G.
AU - Cho-Chung, Yoon S.
PY - 2002/11/26
Y1 - 2002/11/26
N2 - Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.
AB - Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.
UR - http://www.scopus.com/inward/record.url?scp=0037180546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037180546&partnerID=8YFLogxK
U2 - 10.1073/pnas.242617799
DO - 10.1073/pnas.242617799
M3 - Article
C2 - 12438686
AN - SCOPUS:0037180546
VL - 99
SP - 15626
EP - 15631
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 24
ER -