A genomic-scale view of the camp response element-enhancer decoy: A tumor target-based genetic tool

Yee Sook Cho, Meyoung Kon Kim, Chris Cheadle, Catherine Neary, Yun Gyu Park, Kevin G. Becker, Yoon S. Cho-Chung

    Research output: Contribution to journalArticlepeer-review

    24 Citations (Scopus)

    Abstract

    Enhancer DNA decoy oligodeoxynucleotides (ODNs) inhibit transcription by competing for transcription factors. A decoy ODN composed of the cAMP response element (CRE) inhibits CRE- directed gene transcription and tumor growth without affecting normal cell growth. Here, we use DNA microarrays to analyze the global effects of the CRE-decoy ODN in cancer cell lines and in tumors grown in nude mice. The CRE-decoy up-regulates the AP-2β transcription factor gene in tumors but not in the livers of host animals. The up-regulated expression of AP-2β is clustered with the up-regulation of other genes involved in development and cell differentiation. Concomitantly, another cluster of genes involved in cell proliferation and transformation is down-regulated. The observed alterations indicate that CRE-directed transcription favors tumor growth. The CRE-decoy ODN, therefore, may serve as a target-based genetic tool to treat cancer and other diseases in which CRE-directed transcription is abnormally used.

    Original languageEnglish
    Pages (from-to)15626-15631
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume99
    Issue number24
    DOIs
    Publication statusPublished - 2002 Nov 26

    ASJC Scopus subject areas

    • General

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