A gold nanorod-based optical DNA biosensor for the diagnosis of pathogens

Harshala J. Parab, Cheulhee Jung, Joo Hyung Lee, Hyun Gyu Park

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

A novel optical biosensor for detecting target DNA, utilizing gold nanorods (GNRs) as molecular probes is demonstrated. This sensor is based on simultaneous biorecognition-mediated hybridization of target DNA in a sandwich type manner with two different capture probe DNA sequences modified separately with identical sets of GNRs, which leads to aggregation of GNRs. The hybridization induced aggregation as revealed by TEM analysis, promotes the modulation of surface plasmon resonance of GNRs, which forms the basis of complementary target DNA detection from the Chlamydia trachomatis pathogen. Thermally induced reversible dissociation of hybridized DNA is demonstrated by melting analysis. The present sensing strategy is successfully demonstrated by detecting PCR amplified C. trachomatis pathogen gene isolated from human urine sample in a concentration range of 0.25-20. nM. Furthermore, this sensor displays excellent specificity by discriminating the target DNA versus other non-specific pathogenic genes.

Original languageEnglish
Pages (from-to)667-673
Number of pages7
JournalBiosensors and Bioelectronics
Volume26
Issue number2
DOIs
Publication statusPublished - 2010 Oct 1
Externally publishedYes

Fingerprint

Nanotubes
Biosensing Techniques
Pathogens
Nanorods
Biosensors
Gold
DNA
Chlamydia trachomatis
Molecular Probes
Agglomeration
Genes
Surface Plasmon Resonance
Freezing
DNA sequences
Sensors
Surface plasmon resonance
Complementary DNA
Urine
Melting
Polymerase Chain Reaction

Keywords

  • Chlamydia trachomatis
  • DNA biosensor
  • Gold nanorods
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

A gold nanorod-based optical DNA biosensor for the diagnosis of pathogens. / Parab, Harshala J.; Jung, Cheulhee; Lee, Joo Hyung; Park, Hyun Gyu.

In: Biosensors and Bioelectronics, Vol. 26, No. 2, 01.10.2010, p. 667-673.

Research output: Contribution to journalArticle

Parab, Harshala J. ; Jung, Cheulhee ; Lee, Joo Hyung ; Park, Hyun Gyu. / A gold nanorod-based optical DNA biosensor for the diagnosis of pathogens. In: Biosensors and Bioelectronics. 2010 ; Vol. 26, No. 2. pp. 667-673.
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