A green fluorescent protein-based whole-cell bioreporter for the detection of phenylacetic acid

Juhyun Kim, Che Ok Jeon, Woojun Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Phenylacetic acid (PAA) is produced by many bacteria as an antifungal agent and also appears to be an environmentally toxic chemical. The object of this study was to detect PAA using Pseudomonas putida harboring a reporter plasmid that has a PAA-inducible promoter fused to a green fluorescent protein (GFP) gene. Pseudomonas putida KT2440 was used to construct a green fluorescent protein-based reporter fusion using the paaA promoter region to detect the presence of PAA. The reporter strain exhibited a high level of gfp expression in minimal medium containing PAA; however, the level of GFP expression diminished when glucose was added to the medium, whereas other carbon sources, such as succinate and pyruvate, showed no catabolic repression. Interestingly, overexpression of a paaF gene encoding PAA-CoA ligase minimized catabolic repression. The reporter strain could also successfully detect PAA produced by other PAA-producing bacteria. This GFP-based bioreporter provides a useful tool for detecting bacteria producing PAA.

Original languageEnglish
Pages (from-to)1727-1732
Number of pages6
JournalJournal of microbiology and biotechnology
Volume17
Issue number10
Publication statusPublished - 2007 Oct

Keywords

  • Biodegradation
  • Biosensor
  • Catabolic repression
  • GFP
  • Transcriptional regulation

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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