A high glucose concentration stimulates the expression of monocyte chemotactic peptide 1 in human mesangial cells

Chun Gyoo Ihm, Jae Kyung Park, Seong Pyo Hong, Tae Won Lee, Byoung Soo Cho, Myung Jae Kim, Dae Ryong Cha, Hunjoo Ha

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Abstract

The mechanism of glomerular infiltration of monocytes remains unknown in diabetic nephropathy. We examined the effect of a high glucose concentration on monocyte chemotactic peptide 1 (MCP-1) expression in human mesangial cells (MCs) by using enzyme-linked immunosorbent assay and reverse transcription coupled with polymerase chain reaction (PCR). More than a 50% increase in the MCP-1 protein production was observed in MCs cultured in high-glucose medium (450 mg/dl) as compared to normal glucose (100 mg/dl; 1496 ± 75 vs. 966 ± 15 pg/ml after 24 h, 1910 ± 93 vs. 1250 ± 55 pg/ml after 48 h), Semiquantitative PCR showed that phorbol myristate acetate (100 nM) increased the ratio of PCR products for MCP-1 to housekeeping gene glyceraldehyde-3-phosphate dehydrogenase on densitometric results at 24 h by 2.7-fold, which was prevented by calphostin C (200 nM) pretreatment. High glucose increased the ratio by 3-fold as compared to normal glucose at 24 h (0.72 ± 0.11 vs. 0.24 ± 0.01). This was also suppressed by calphostin C pretreatment. These findings demonstrate that high glucose can directly increase MCP-1 expression in MCs, which may contribute to monocyte infiltration in diabetic nephropathy, and this is regulated by protein kinase C.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalNephron
Volume79
Issue number1
DOIs
Publication statusPublished - 1998 Jan 1

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Keywords

  • High-glucose medium
  • Mesangial cells
  • Monocyte chemotactic peptide 1
  • Protein kinase C

ASJC Scopus subject areas

  • Physiology
  • Nephrology
  • Physiology (medical)
  • Urology

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