A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Doyeon Kim; Sukjun Kim; Joori Park; Hee Ryung Chang; Jeeyoon Chang; Junhak Ahn; Heedo Park; Junehee Park; Narae Son; Gihyeon Kang; Jeonghun Kim; Kisoon Kim; Man Seong Park; Yoon Ki Kim; Daehyun Baek

doi:10.1038/s41467-021-25361-5

A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Doyeon Kim, Sukjun Kim, Joori Park, Hee Ryung Chang, Jeeyoon Chang, Junhak Ahn, Heedo Park, Junehee Park, Narae Son, Gihyeon Kang, Jeonghun Kim, Kisoon Kim, Man Seong Park, Yoon Ki Kim, Daehyun Baek

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.

Original language	English
Article number	5120
Journal	Nature communications
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-25361-5
Publication status	Published - 2021 Dec 1

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)

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10.1038/s41467-021-25361-5

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@article{01da90719655492bb1544dd616c40cd5,

title = "A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome",

abstract = "COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.",

author = "Doyeon Kim and Sukjun Kim and Joori Park and Chang, {Hee Ryung} and Jeeyoon Chang and Junhak Ahn and Heedo Park and Junehee Park and Narae Son and Gihyeon Kang and Jeonghun Kim and Kisoon Kim and Park, {Man Seong} and Kim, {Yoon Ki} and Daehyun Baek",

note = "Funding Information: This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT, Republic of Korea (NRF-2014M3C9A3063541, NRF-2015R1A3A2033665, NRF-2017M3A9E4061995, NRF-2018R1A5A1024261, NRF-2018M3A9H4056537, NRF-2019M3E5D3073104, NRF-2020R1A2C3007032, and NRF-2020R1A5A1018081) and from Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (HI15C3224). Publisher Copyright: {\textcopyright} 2021, The Author(s).",

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doi = "10.1038/s41467-021-25361-5",

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AU - Kim, Doyeon

AU - Kim, Sukjun

AU - Park, Joori

AU - Chang, Hee Ryung

AU - Chang, Jeeyoon

AU - Ahn, Junhak

AU - Park, Heedo

AU - Park, Junehee

AU - Son, Narae

AU - Kang, Gihyeon

AU - Kim, Jeonghun

AU - Kim, Kisoon

AU - Park, Man Seong

AU - Kim, Yoon Ki

AU - Baek, Daehyun

N1 - Funding Information: This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT, Republic of Korea (NRF-2014M3C9A3063541, NRF-2015R1A3A2033665, NRF-2017M3A9E4061995, NRF-2018R1A5A1024261, NRF-2018M3A9H4056537, NRF-2019M3E5D3073104, NRF-2020R1A2C3007032, and NRF-2020R1A5A1018081) and from Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (HI15C3224). Publisher Copyright: © 2021, The Author(s).

PY - 2021/12/1

Y1 - 2021/12/1

N2 - COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.

AB - COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.

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JO - Nature Communications

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ER -

A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Abstract

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