A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome

Doyeon Kim, Sukjun Kim, Joori Park, Hee Ryung Chang, Jeeyoon Chang, Junhak Ahn, Heedo Park, Junehee Park, Narae Son, Gihyeon Kang, Jeonghun Kim, Kisoon Kim, Man Seong Park, Yoon Ki Kim, Daehyun Baek

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

COVID-19 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which infected >200 million people resulting in >4 million deaths. However, temporal landscape of the SARS-CoV-2 translatome and its impact on the human genome remain unexplored. Here, we report a high-resolution atlas of the translatome and transcriptome of SARS-CoV-2 for various time points after infecting human cells. Intriguingly, substantial amount of SARS-CoV-2 translation initiates at a novel translation initiation site (TIS) located in the leader sequence, termed TIS-L. Since TIS-L is included in all the genomic and subgenomic RNAs, the SARS-CoV-2 translatome may be regulated by a sophisticated interplay between TIS-L and downstream TISs. TIS-L functions as a strong translation enhancer for ORF S, and as translation suppressors for most of the other ORFs. Our global temporal atlas provides compelling insight into unique regulation of the SARS-CoV-2 translatome and helps comprehensively evaluate its impact on the human genome.

Original languageEnglish
Article number5120
JournalNature communications
Volume12
Issue number1
DOIs
Publication statusPublished - 2021 Dec

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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