A high-throughput microfluidic assay to study neurite response to growth factor gradients

Chandrasekhar R. Kothapalli, Ed Van Veen, Sarra De Valence, Seok Chung, Ioannis K. Zervantonakis, Frank B. Gertler, Roger D. Kamm

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Studying neurite guidance by diffusible or substrate bound gradients is challenging with current techniques. In this study, we present the design, fabrication and utility of a microfluidic device to study neurite guidance under chemogradients. Experimental and computational studies demonstrated the establishment of a steep gradient of guidance cue within 30 min and stable for up to 48 h. The gradient was found to be insensitive to external perturbations such as media change and movement of device. The effects of netrin-1 (0.1-10 g mL-1) and brain pulp (0.1 L mL-1) were evaluated for their chemoattractive potential on neurite turning, while slit-2 (62.5 or 250 ng mL-1) was studied for its chemorepellant properties. Hippocampal or dorsal root ganglion (DRG) neurons were seeded into a micro-channel and packed onto the surface of a 3D collagen gel. Neurites grew into the matrix in three dimensions, and a gradient of guidance cue was created orthogonal to the direction of neurite growth to impact guidance. The average turning angle of each neurite was measured and averaged across multiple devices cultured under similar conditions to quantify the effect of guidance cue gradient. Significant positive turning towards gradient was measured in the presence of brain pulp and netrin-1 (1 g mL-1), relative to control cultures which received no external guidance cue (p < 0.001). Netrin-1 released from transfected fibroblasts had the most positive turning effect of all the chemoattractive cues tested (p < 0.001). Slit-2 exhibited strong chemorepellant characteristics on both hippocampal and DRG neurite guidance at 250 ng mL-1 concentration. Slit-2 also showed similar behavior on DRG neuron invasion into 3D collagen gel (p < 0.01 relative to control cultures). Taken together, the results suggest the utility of this microfluidic device to generate stable chemogradients for studying neurobiology, cell migration and proliferation, matrix remodeling and co-cultures with other cell lines, with potential applications in cancer biology, tissue engineering and regenerative medicine.

Original languageEnglish
Pages (from-to)497-507
Number of pages11
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume11
Issue number3
DOIs
Publication statusPublished - 2011 Feb 7

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Microfluidics
Neurites
Cues
Lab-On-A-Chip Devices
Assays
Intercellular Signaling Peptides and Proteins
Throughput
Spinal Ganglia
Collagen
Neurons
Pulp
Brain
Gels
Bioelectric potentials
Fibroblasts
Equipment and Supplies
Tissue engineering
Cell culture
Regenerative Medicine
Neurobiology

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Kothapalli, C. R., Van Veen, E., De Valence, S., Chung, S., Zervantonakis, I. K., Gertler, F. B., & Kamm, R. D. (2011). A high-throughput microfluidic assay to study neurite response to growth factor gradients. Lab on a Chip - Miniaturisation for Chemistry and Biology, 11(3), 497-507. https://doi.org/10.1039/c0lc00240b

A high-throughput microfluidic assay to study neurite response to growth factor gradients. / Kothapalli, Chandrasekhar R.; Van Veen, Ed; De Valence, Sarra; Chung, Seok; Zervantonakis, Ioannis K.; Gertler, Frank B.; Kamm, Roger D.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 11, No. 3, 07.02.2011, p. 497-507.

Research output: Contribution to journalArticle

Kothapalli, CR, Van Veen, E, De Valence, S, Chung, S, Zervantonakis, IK, Gertler, FB & Kamm, RD 2011, 'A high-throughput microfluidic assay to study neurite response to growth factor gradients', Lab on a Chip - Miniaturisation for Chemistry and Biology, vol. 11, no. 3, pp. 497-507. https://doi.org/10.1039/c0lc00240b
Kothapalli, Chandrasekhar R. ; Van Veen, Ed ; De Valence, Sarra ; Chung, Seok ; Zervantonakis, Ioannis K. ; Gertler, Frank B. ; Kamm, Roger D. / A high-throughput microfluidic assay to study neurite response to growth factor gradients. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2011 ; Vol. 11, No. 3. pp. 497-507.
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