A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

Ji Young Eum, Hwang Sang Youn, Youngjun Ju, Jong Min Shim, Yunxian Piao, Jinwoo Lee, Hak Sung Kim, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.

Original languageEnglish
Pages (from-to)3546-3548
Number of pages3
JournalChemical Communications
Volume50
Issue number27
DOIs
Publication statusPublished - 2014 Mar 6

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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