A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria

Tae Hyoung Yoo, Chang Jin Park, Gil Je Lee, Ryoung Shin, Ji Hyun Yun, Ki Jeong Kim, Ki Hyeong Rhee, Kyung Hee Paek

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Capsicum annuum L. is infected by a number of viruses, including the tobacco mosaic virus (TMV). To study the defense-related genes that are induced by TMV in hot peppers, the pepper plant, which is susceptible to P1.2 but resistant to the P0 pathotype of TMV, was inoculated with TMV-P0. Differential screening isolated the genes that were specifically up- or down-regulated during the hypersensitive response (HR). The CaAPX1 cDNA clone that putatively encodes a polypeptide of cytosolic ascorbate peroxidase was selected as an up-regulated gene. It was isolated for further study. The full-length cDNA for CaAPX1, which is 972 bp long, contained the open-reading frame of 250-amino acid residues. A genomic Southern blot analysis showed that there were only limited copies of the CaAPX1 gene in the hot pepper genome. In hot pepper cv. Bugang, which is resistant to TMV-P0 and susceptible to TMV-P1.2, the CaAPX1 gene transcript was accumulated by TMV-P0, but not by TMV-P1.2 inoculation. CaAPX1 transcripts began to accumulate 24 h post-inoculation of TMV-P0, and increased gradually until 96 h. To investigate whether each transcript is induced by other stimuli, the plants were treated with various chemicals and wounding. A striking induction of the CaAPX1 transcript was observed at 2 h. It subsided 12 h after salicylic acid (SA), ethephon, and methyl jasmonate (MeJA) treatments. The response of the gene upon other pathogen infection was also examined by a bacterial pathogen (Xanthomonas campestris pv. vesicatoria race 3) inoculation. The CaAPX1 gene was induced in a hot pepper (C annuum cv. ECW 20R) that was resistant to this bacterial pathogen, but not in a susceptible hot pepper (C. annuum cv. ECW). These results suggest the possible role(s) for the CaAPX1 gene in plant defense against viral and bacterial pathogen.

Original languageEnglish
Pages (from-to)75-84
Number of pages10
JournalMolecules and Cells
Volume14
Issue number1
Publication statusPublished - 2002 Aug 1

Fingerprint

Ascorbate Peroxidases
Tobacco Mosaic Virus
Capsicum
Complementary DNA
Viruses
Bacteria
Genes
Xanthomonas campestris
Plant Genes
Salicylic Acid
Southern Blotting
Open Reading Frames
Clone Cells
Genome
Amino Acids

Keywords

  • Ascorbate Peroxidase cDNA Clone
  • Differential Screening
  • Hot Pepper (Capsicum annuum L.)
  • Hypersensitive Response
  • Tobacco Mosaic Virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria. / Yoo, Tae Hyoung; Park, Chang Jin; Lee, Gil Je; Shin, Ryoung; Yun, Ji Hyun; Kim, Ki Jeong; Rhee, Ki Hyeong; Paek, Kyung Hee.

In: Molecules and Cells, Vol. 14, No. 1, 01.08.2002, p. 75-84.

Research output: Contribution to journalArticle

Yoo, TH, Park, CJ, Lee, GJ, Shin, R, Yun, JH, Kim, KJ, Rhee, KH & Paek, KH 2002, 'A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria', Molecules and Cells, vol. 14, no. 1, pp. 75-84.
Yoo, Tae Hyoung ; Park, Chang Jin ; Lee, Gil Je ; Shin, Ryoung ; Yun, Ji Hyun ; Kim, Ki Jeong ; Rhee, Ki Hyeong ; Paek, Kyung Hee. / A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria. In: Molecules and Cells. 2002 ; Vol. 14, No. 1. pp. 75-84.
@article{9648ae475917431bab3382f7eaa512dc,
title = "A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria",
abstract = "Capsicum annuum L. is infected by a number of viruses, including the tobacco mosaic virus (TMV). To study the defense-related genes that are induced by TMV in hot peppers, the pepper plant, which is susceptible to P1.2 but resistant to the P0 pathotype of TMV, was inoculated with TMV-P0. Differential screening isolated the genes that were specifically up- or down-regulated during the hypersensitive response (HR). The CaAPX1 cDNA clone that putatively encodes a polypeptide of cytosolic ascorbate peroxidase was selected as an up-regulated gene. It was isolated for further study. The full-length cDNA for CaAPX1, which is 972 bp long, contained the open-reading frame of 250-amino acid residues. A genomic Southern blot analysis showed that there were only limited copies of the CaAPX1 gene in the hot pepper genome. In hot pepper cv. Bugang, which is resistant to TMV-P0 and susceptible to TMV-P1.2, the CaAPX1 gene transcript was accumulated by TMV-P0, but not by TMV-P1.2 inoculation. CaAPX1 transcripts began to accumulate 24 h post-inoculation of TMV-P0, and increased gradually until 96 h. To investigate whether each transcript is induced by other stimuli, the plants were treated with various chemicals and wounding. A striking induction of the CaAPX1 transcript was observed at 2 h. It subsided 12 h after salicylic acid (SA), ethephon, and methyl jasmonate (MeJA) treatments. The response of the gene upon other pathogen infection was also examined by a bacterial pathogen (Xanthomonas campestris pv. vesicatoria race 3) inoculation. The CaAPX1 gene was induced in a hot pepper (C annuum cv. ECW 20R) that was resistant to this bacterial pathogen, but not in a susceptible hot pepper (C. annuum cv. ECW). These results suggest the possible role(s) for the CaAPX1 gene in plant defense against viral and bacterial pathogen.",
keywords = "Ascorbate Peroxidase cDNA Clone, Differential Screening, Hot Pepper (Capsicum annuum L.), Hypersensitive Response, Tobacco Mosaic Virus",
author = "Yoo, {Tae Hyoung} and Park, {Chang Jin} and Lee, {Gil Je} and Ryoung Shin and Yun, {Ji Hyun} and Kim, {Ki Jeong} and Rhee, {Ki Hyeong} and Paek, {Kyung Hee}",
year = "2002",
month = "8",
day = "1",
language = "English",
volume = "14",
pages = "75--84",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

TY - JOUR

T1 - A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria

AU - Yoo, Tae Hyoung

AU - Park, Chang Jin

AU - Lee, Gil Je

AU - Shin, Ryoung

AU - Yun, Ji Hyun

AU - Kim, Ki Jeong

AU - Rhee, Ki Hyeong

AU - Paek, Kyung Hee

PY - 2002/8/1

Y1 - 2002/8/1

N2 - Capsicum annuum L. is infected by a number of viruses, including the tobacco mosaic virus (TMV). To study the defense-related genes that are induced by TMV in hot peppers, the pepper plant, which is susceptible to P1.2 but resistant to the P0 pathotype of TMV, was inoculated with TMV-P0. Differential screening isolated the genes that were specifically up- or down-regulated during the hypersensitive response (HR). The CaAPX1 cDNA clone that putatively encodes a polypeptide of cytosolic ascorbate peroxidase was selected as an up-regulated gene. It was isolated for further study. The full-length cDNA for CaAPX1, which is 972 bp long, contained the open-reading frame of 250-amino acid residues. A genomic Southern blot analysis showed that there were only limited copies of the CaAPX1 gene in the hot pepper genome. In hot pepper cv. Bugang, which is resistant to TMV-P0 and susceptible to TMV-P1.2, the CaAPX1 gene transcript was accumulated by TMV-P0, but not by TMV-P1.2 inoculation. CaAPX1 transcripts began to accumulate 24 h post-inoculation of TMV-P0, and increased gradually until 96 h. To investigate whether each transcript is induced by other stimuli, the plants were treated with various chemicals and wounding. A striking induction of the CaAPX1 transcript was observed at 2 h. It subsided 12 h after salicylic acid (SA), ethephon, and methyl jasmonate (MeJA) treatments. The response of the gene upon other pathogen infection was also examined by a bacterial pathogen (Xanthomonas campestris pv. vesicatoria race 3) inoculation. The CaAPX1 gene was induced in a hot pepper (C annuum cv. ECW 20R) that was resistant to this bacterial pathogen, but not in a susceptible hot pepper (C. annuum cv. ECW). These results suggest the possible role(s) for the CaAPX1 gene in plant defense against viral and bacterial pathogen.

AB - Capsicum annuum L. is infected by a number of viruses, including the tobacco mosaic virus (TMV). To study the defense-related genes that are induced by TMV in hot peppers, the pepper plant, which is susceptible to P1.2 but resistant to the P0 pathotype of TMV, was inoculated with TMV-P0. Differential screening isolated the genes that were specifically up- or down-regulated during the hypersensitive response (HR). The CaAPX1 cDNA clone that putatively encodes a polypeptide of cytosolic ascorbate peroxidase was selected as an up-regulated gene. It was isolated for further study. The full-length cDNA for CaAPX1, which is 972 bp long, contained the open-reading frame of 250-amino acid residues. A genomic Southern blot analysis showed that there were only limited copies of the CaAPX1 gene in the hot pepper genome. In hot pepper cv. Bugang, which is resistant to TMV-P0 and susceptible to TMV-P1.2, the CaAPX1 gene transcript was accumulated by TMV-P0, but not by TMV-P1.2 inoculation. CaAPX1 transcripts began to accumulate 24 h post-inoculation of TMV-P0, and increased gradually until 96 h. To investigate whether each transcript is induced by other stimuli, the plants were treated with various chemicals and wounding. A striking induction of the CaAPX1 transcript was observed at 2 h. It subsided 12 h after salicylic acid (SA), ethephon, and methyl jasmonate (MeJA) treatments. The response of the gene upon other pathogen infection was also examined by a bacterial pathogen (Xanthomonas campestris pv. vesicatoria race 3) inoculation. The CaAPX1 gene was induced in a hot pepper (C annuum cv. ECW 20R) that was resistant to this bacterial pathogen, but not in a susceptible hot pepper (C. annuum cv. ECW). These results suggest the possible role(s) for the CaAPX1 gene in plant defense against viral and bacterial pathogen.

KW - Ascorbate Peroxidase cDNA Clone

KW - Differential Screening

KW - Hot Pepper (Capsicum annuum L.)

KW - Hypersensitive Response

KW - Tobacco Mosaic Virus

UR - http://www.scopus.com/inward/record.url?scp=0037206314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037206314&partnerID=8YFLogxK

M3 - Article

C2 - 12243356

AN - SCOPUS:0037206314

VL - 14

SP - 75

EP - 84

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -